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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 83 results for: REV

Showing: 1 - 20 of 83 results

  1. Lentivirus Plasmids

    Type
    Collection
    ...along with a plasmid encoding rev . Didier Trono 12253 pRSV-Rev 3rd Contains rev . Can be used with 3rd generation...Expresses gag-pol and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd Contains gag , pol , rev , tat , vif , and...Didier Trono 12260 psPAX2 2nd Contains gag , pol , rev , and tat . Can be used with 2nd and 3rd generation...Trono 12263 pCMV delta R8.2 2nd Contains gag , pol , rev , and tat . Can be used with 2nd and 3rd generation...17531 pCD/NL-BH*DDD 2nd Contains gag-pol , tat , and rev . Use with envelope plasmids from Jakob Reiser. Jakob...Sergey Kasparov 22500 pNHP 2nd Contains gag , pol , rev , and tat . Sergey Kasparov Return to top Popular...of pLL3.7 with added genetic elements known to prevent epigenetic silencing. Expresses shRNA under mouse...

  2. p53 Pathway

    Type
    Collection
    ...suppression. Bieging KT, Mello SS, Attardi LD. Nat Rev Cancer. 2014 May;14(5):359-70. PubMed PMID: 24739573...from the mutant p53 field. Brosh R, Rotter V. Nat Rev Cancer. 2009 Oct;9(10):701-13. PubMed PMID: 19693097...p53 targets. Menendez D, Inga A, Resnick MA. Nat Rev Cancer. 2009 Oct;9(10):724-37. PubMed PMID: 19776742...

  3. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...AAV9 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-9-18917P 18917-AAV9 AAV-FLEX-rev-ChR2-tdtomato... James M. Wilson AV-1-18917P 18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Optogenetics Scott Sternson AV-1-20071P...Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-1-20949P 20949...GENIE, Douglas Kim AV-10-18917P 18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-10-PV1963 105542-... James M. Wilson AV-5-18917P 18917-AAV5 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-5-20071P 20071-AAV5...Pennsylvania Vector Core (Penn Vector Core) and Addgene previously partnered to provide high-quality AAV vectors...

  4. mTOR Pathway

    Type
    Collection
    ... L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov. 2015 Apr;14(4):261-78. doi: 10.1038/...

  5. Chemogenetics AAV Preps

    Type
    Collection
    ...PHPeB Jordane Dimidschstein 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent...

  6. Viral Production

    Type
    Collection
    ...probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease... et al. (2014) (Link opens in a new window) ). Previous experience suggests that titers obtained using...

  7. Ras Pathway

    Type
    Collection
    ...signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763...

  8. Rett Syndrome

    Type
    Collection
    ...biological progress over 50 years in Rett syndrome. Nat Rev Neurol . 13, 37–51. PMID: 27934853 Neul et al. 2008...RNA trans-splicing, and protein replacement to reverse the impacts of this disease. Addgene is working...typically between 18 to 30 months, is regression of previously acquired skills, notably loss of acquired purposeful... demonstrates that Rett syndrome symptoms are reversible in mice if MECP2 gene expression is switched ... a new window) PMID: 24399845 Guy et al. 2007. Reversal of Neurological Defects in a Mouse Model of Rett...Laurvick et al. 2006. Rett syndrome in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352...PMID: 18337588 Neul et al. 2010. Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...evolutionary classification of CRISPR-Cas systems. Nat Rev Microbiol . 13(11):722-36. PMID: 26411297 Mali P,...gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing down. This prokaryotic...employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas complex from binding to DNA. Others...or repressors can modulate transcription in a reversible manner. Epigenetics : dCas9 fused to epigenetic...Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish Xenopus References Barrangou...

  10. Validated gRNA Sequences

    Type
    Collection
    ... cerevisiae CTAGATATTAAAATGTCTAA 64379 activate S. pyogenes 23977949 Lu CYC1m promoter S. cerevisiae GTTGAAAGTATTAGTTAAAG...70655 cut S. pyogenes 26472758 Sabatini CAN1 S. cerevisiae GATACGTTCTCTATGGAGGA 43803 cut S. pyogenes 23460208...interfere S. pyogenes 23849981 Qi CYC1m promoter S. cerevisiae ACAGAGCACATGCATGCCAT 64385 activate S. pyogenes...pyogenes 23977949 Lu CYC1m promoter S. cerevisiae ACTAATACTTTCAACATTTT 64387 activate S. pyogenes 23977949...23977949 Lu CYC1m promoter S. cerevisiae ATATCGAATTCCTGCAGCCC 64382 activate S. pyogenes 23977949 Lu CYC1m promoter... promoter S. cerevisiae ATATTCTTTCCTTATACATT 64380 activate S. pyogenes 23977949 Lu CYC1m promoter S. ...activate S. pyogenes 23977949 Lu CYC1m promoter S. cerevisiae TACATACAGTAGGATCCTA 64381 activate S. pyogenes...

  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Khakh ATP FRET-based ATP sensor ATeam1.03-nD/nA Reversible dimerization of Aequorea victoria fluorescent...patterning of cGMP in vascular smooth muscle cells revealed by single GFP-linked biosensors. Proc Natl Acad...Peredox-mCherry in plants In Vivo NADH/NAD+ Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism in Plants...for pyruvate A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate...pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron. 2009 Feb 12...macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. J Cell Biol. 2010 Feb...for pH sensing The zebrafish merovingian mutant reveals a role for pH regulation in hair cell toxicity ...

  12. Brain Initiative Collection

    Type
    Collection
    ...pAAV-EF1a-iCreV can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase...pAAV-EF1a-iDreV can be used to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase...Neurotechnologies® (BRAIN) Initiative aims to revolutionize our knowledge of the human brain through the... behavior. Long desired by researchers, the revolutionary dynamic picture of the brain generated through...will provide novel ways to treat, cure, and even prevent brain disorders. The BRAIN Initiative supports ...

  13. Open Enzyme Collection

    Type
    Collection
    ...Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes You...DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These ...reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and dNTP synthesis...Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes DNA...pOpen-XbaIR XbaI 165568 pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546...pOpen-MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations... (lack H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) ...

  14. CRISPR Guide

    Type
    Collection
    ...the donor template, incorporating mutations that prevent further Cas9 cleavage will improve the accuracy... method in which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the C-terminus...the desired edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces ...editing. PASTE uses a CRISPR-Cas9n fused to both a reverse-transcriptase and a serine integrase (Bxb1), along...dCas9-mediated gene activation or repression is reversible, as it does not permanently modify the genomic...multiple fusions. The effects of CRISPRi/a may be reversible once the effector is inactivated or removed from... and even cell differentiation. The system is reversible, with a companion CRISPRon to undo repression...

  15. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...search and replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of...the desired edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces ... use the Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and...editing, including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools...additional protection added to 3’ tail of pegRNA to prevent RNA degradation For more in-depth information on...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

  16. Caltech Systemic Capsids

    Type
    Collection
    ...140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible...strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory...check out Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please...light-inducible recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...most popular choices for rapid, efficient, and reversible control of gene expression in eukaryotes. To ...VP16 transcriptional activation domain); rtTA: reverse tetracycline-controlled transactivator (fusion ...tetracycline-dependent repression. Mutating these residues led to a reverse Tet repressor, or rTetR, which specifically binds...the TRE in the presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA) was...TetR-tetO system, although the DNA-binding profile is reversed (Figure 1). However, rtTA can induce stronger ... marker. Aviv Regev , Joan Brugge Content last reviewed 20 June 2025. References Das, A. T., Tenenbaum...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG...of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors...Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN kits - Construct ... and expression of bacterial gene clusters S. cerevisiae plasmids for multiple marker selection TRP1 Yeast...InvivoGen trademark. Return to top Content last reviewed 28 May 2025. Do you have suggestions for other...

  19. Zhang Lab CRISPR Page

    Type
    Collection
    ... plasmid can be digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed...FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  20. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https://doi.org/10.1371... fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org/10.1002...selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https://doi.org/10.1186...24161108 (Link opens in a new window) Content last reviewed: 12 August 2025...
Showing: 1 - 20 of 83 results