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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,508 results for: crispr

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Showing: 3361 - 3380 of 25508 results

  1. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid
    #208645
    Purpose
    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    Use
    CRISPR; Ssdna donnor preparation for knock-in of…
    Tags
    Twin Strep
    Mutation
    Two SNP (rs749555789, rs760194863) were introduce…
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208645-plasmid-map-sequence-id-415926

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  12. pLenti-CRISPRv2-sgENPP1_#2-hygro

    Plasmid
    #258010
    Purpose
    ENPP1 sgRNA with Cas9 and hygromycin resistance
    Depositor
    Brian Ruffell
    Insert
    sgRNA with Cas9 and hygroymycin resistance (Enpp1 Mouse)
    Use
    CRISPR and Lentiviral
    Available since
    Aug. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    258010-plasmid-map-sequence-id-515883

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  13. pLenti-CRISPRv2-sgENPP1_#1-hygro

    Plasmid
    #258011
    Purpose
    ENPP1 sgRNA with Cas9 and hygromycin resistance
    Depositor
    Brian Ruffell
    Insert
    sgRNA with Cas9 and hygromycin resistance (Enpp1 Mouse)
    Use
    CRISPR and Lentiviral
    Available since
    Aug. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    258011-plasmid-map-sequence-id-515884

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  15. pLEX307_CR-PDHA2S291D/S293D

    Plasmid
    #115202
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Publication
    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    115202-plasmid-map-sequence-id-231793

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  16. MTK0_046

    Plasmid
    #123976
    Purpose
    Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hThal5.10-2_hg18 CAS9 Destination - HygroR
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123976-plasmid-map-sequence-id-251798

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Showing: 3361 - 3380 of 25508 results