We narrowed to 23,628 results for: crispr

1. RRM1 E2.3 gRNA

   Plasmid

   #90881

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RRM1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   RRM1 (Guide Designation E2.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. RRM2 E4.3 gRNA

   Plasmid

   #90883

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RRM2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   RRM2 (Guide Designation E4.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. RRM2 E3.3 gRNA

   Plasmid

   #90882

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RRM2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   RRM2 (Guide Designation E3.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. BRCA2 A12.2 gRNA

   Plasmid

   #90551

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A12.2)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK2139

   Plasmid

   #65776

   Purpose

   Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. c3GIC9

   Plasmid

   #62191

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. EZH2 E9.3 gRNA

   Plasmid

   #90684

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EZH2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   EZH2 (Guide Designation E9.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI Cas9 H2B-RFP

   Plasmid

   #92397

   Purpose

   CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AsdCas12a VPR

   Plasmid

   #188512

   Purpose

   Expresses FLAG tagged AsdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. VCP F12.4 gRNA

    Plasmid

    #90939

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F12.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. VCP F11.4 gRNA

    Plasmid

    #90938

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F11.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BE-VQRCas9

    Plasmid

    #101740

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, VQR mutations

    Promoter

    CMV

    Available Since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-SMVP-Cas9N-U6-gRNA M3

    Plasmid

    #80936

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9S(RBSmut)_Psyn-sgRNA500

    Plasmid

    #149576

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EZH2 E10.3 gRNA

    Plasmid

    #90685

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EZH2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    EZH2 (Guide Designation E10.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-T3-CjCAS9-pA

    Plasmid

    #131543

    Purpose

    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    FLAG-SV40NLS, SV40NLS, and polyA

    Expression

    Mammalian

    Mutation

    Human codon-optimized

    Promoter

    CAG, T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6T7

    Plasmid

    #71462

    Purpose

    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

    Depositor

    Willie Mark

    Article

    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    human U6/phage T7

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTU422

    Plasmid

    #178982

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Inserts

    gRAMP
    CRISPR array
    

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MASTL B4.4 gRNA

    Plasmid

    #90755

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MASTL

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MASTL (Guide Designation B4.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge