We narrowed to 12,697 results for: ef1a

1. Lenti:FlipGFP-T2A-mCherry

   Plasmid

   #223198

   Purpose

   Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

   Depositor

   Yeh-Hsing Lao

   Article

   Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

   Insert

   FlipGFP-T2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_174

   Plasmid

   #136476

   Purpose

   Lentiviral expression of EnAsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   EnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p65-mNeonGreen

   Plasmid

   #127172

   Purpose

   Fluorescently tagged p65 reporter

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   GFP-RelA (RELA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXL004-ishRNA-scramble

   Plasmid

   #36311

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of scramble
   

   Use

   Lentiviral and RNAi

   Tags

   EF1a-TetR-IRES-RFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-H1

   Available since

   July 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti_MCP-p300_Hygro

   Plasmid

   #138459

   Purpose

   lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   p300 core
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TO-ASCL1-DLX2

   Plasmid

   #182307

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   Ascl1 (Ascl1 Mouse)
   Dlx2 (Dlx2 Mouse)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pTRE3G-bidirectional

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-RT001-LVB

   Plasmid

   #163381

   Purpose

   Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   LaG17-VEEGFRtm-BFP (LVB)
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

   Plasmid

   #190111

   Purpose

   AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   AAV

   Tags

   bpNLS

   Expression

   Mutation

   H840A

   Promoter

   EF1a

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX_HA

    Plasmid

    #178534

    Purpose

    Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX307 LacZ

    Plasmid

    #117730

    Purpose

    Open reading frame vector encoding LacZ

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX311 Luciferase

    Plasmid

    #117735

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX307 Luciferase

    Plasmid

    #117734

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-mito-mTagBFP2

    Plasmid

    #216130

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-Ftractin-mCherry

    Plasmid

    #85131

    Purpose

    lentiviral expression of an F-actin live-cell marker (mCherry)

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBB HA cIAP1

    Plasmid

    #38232

    Depositor

    Colin Duckett

    Article

    Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL056

    Plasmid

    #177168

    Purpose

    Expresses wildtype MRE11 in human cells

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-mAmetrine-CAAX

    Plasmid

    #216132

    Purpose

    Lentiviral vector for expressing mAmetrine localizing to the plasma membrane.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-mAmetrine-CAAX-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid

    #188523

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    3XNLS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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