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pHDR_REC8-T2A-mGreenLantern-puroTK

Plasmid

#222521

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to REC8.

Depositor

George Church

Publication

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

mGreenLantern

Use

CRISPR

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::mCherry

Plasmid

#124348

Purpose

Nuclear Localized Pan-Neuronal mCherry

Depositor

Andrew Leifer

Insert

rab-3, mCherry (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-tax-4P::his-24::mOrange

Plasmid

#124340

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

tax-4, mOrange (tax-4 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-lim-4P::his-24::Cerulean

Plasmid

#124336

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

lim-4, Cerulean (lim-4 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-Streptavidin-RGG

Plasmid

#242451

Purpose

Expresses the MBP-Streptavidin-RGG fusion protein in E Coli.

Depositor

Benjamin Schuster

Publication

Kelley et al Nat Commun. 2025 Apr 14;16(1):3521. doi:

Insert

MBP-Streptavidin-RGG (using LAF-1 RGG domain)

Tags

MBP and Steptavidin

Expression

Bacterial

Available since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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T2A GFP H2B

Plasmid

#227722

Purpose

Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Publication

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)

Use

Cloning vector

Tags

T2A 3xNLS GFP

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pEG459

Plasmid

#40088

Depositor

Geraldine Seydoux

Publication

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S404A) (mex-5 Nematode)

Tags

MBP

Expression

Bacterial

Mutation

S404A

Promoter

P-lac

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pDMG8a

Plasmid

#33179

Depositor

David Bartel

Publication

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

C27H6.9 3'UTR (C27H6.9 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG1a

Plasmid

#33158

Depositor

David Bartel

Publication

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

cog-1 3'UTR (cog-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDP-let-7_LPB

Plasmid

#26560

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPB

Use

Transcription vector

Mutation

loop mutant B

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNAOX-twk46

Plasmid

#16129

Depositor

Larry Salkoff

Publication

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Voltage-gated K+ channel (twk-46 Nematode)

Use

Mammalian expression & oocyte expression

Mutation

N/A

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

stop at beginning of last exon

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mg172

Plasmid

#34652

Depositor

Miriam Goodman

Publication

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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mg182

Plasmid

#34662

Depositor

Miriam Goodman

Publication

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B2;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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U653-GFP ILtoEK

Plasmid

#24473

Depositor

Ron Vale

Publication

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

GFP

Expression

Bacterial

Mutation

I362E/L365K (neck destabilization mutant)

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pJL2

Plasmid

#184130

Purpose

For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

Depositor

Alejandro Aballay

Publication

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmrp-1

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET3CTc3A(1-135)

Plasmid

#63138

Purpose

Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

Depositor

Titia Sixma

Publication

Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

Insert

Tc3A

Tags

6xHis

Expression

Bacterial

Mutation

1-135 aa bipartite domain

Promoter

T7

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pSAH66 - [BAGp | gfp | tbb-2 UTR]

Plasmid

#200340

Purpose

gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Insert

[BAGp | gfp | tbb-2 UTR]

Expression

Worm

Promoter

WBGene00001536, flp-17

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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