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pEGFP-C1_N-NLS_WT POLH

Plasmid

#221868

Purpose

Expresses WT POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_POLH_ΔGG ubiquitin

Plasmid

#221869

Purpose

Expresses GFP-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG ubiquitin

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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SLX4-SFB-D556-637

Plasmid

#247860

Purpose

Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged SLX4 D556-637

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

SLX4 (SLX4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

deletion of amino acid 556-637

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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AcrVA1_AsLOV2-S103ΔELS

Plasmid

#246049

Purpose

Expression of AcrVA1-AsLOV2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables optogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_AsLOV2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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AcrVA1_cpGR2(GGS5)-S103ΔELS

Plasmid

#246050

Purpose

Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables chemogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_cpGR2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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H1_sgRNA(GRIN2B)_SauCas9

Plasmid

#246054

Purpose

Expression of a sgRNA targeting the GRIN2B locus and SauCas9 under the same pol-III H1 promoter

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

SauCas9 and sgRNA(GRIN2B)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIC3_cpGR2(GGS5)-F59

Plasmid

#246053

Purpose

Expression of AcrIIC3-cpGR2 hybrid in mammalian cells; insertion before F59; enables chemogenetic control of NmeCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIC3_cpGR2-F59

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA4_cpGR2(GGS5)-ΔN64_Q65_E66

Plasmid

#246052

Purpose

Expression of AcrIIA4-cpGR2 hybrid in mammalian cells after Bubeck et al Nat Methods 2018; enables chemogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA4_cpGR2-ΔN64/Q65/E66

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔN64/Q65/E66

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246046

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-D41

Plasmid

#246045

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before D41; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_AsLOV2-N77

Plasmid

#246044

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before N77; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_AsLOV2-D41

Plasmid

#246043

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before D41; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-4TO-PHF13W255A

Plasmid

#246124

Purpose

Expression of human PHF13 (W255A) with N terminal tet operator in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13W255A (PHF13 Human)

Expression

Mammalian

Mutation

PHF13W255A

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-4TO-PHF13M246A

Plasmid

#246123

Purpose

Expression of human PHF13 (M246A) with N terminal tet operator in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13M246A (PHF13 Human)

Expression

Mammalian

Mutation

PHF13M246A

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-4TO-PHF13

Plasmid

#246118

Purpose

Expression of human PHF13 with N terminal tet operator in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Expression

Mammalian

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pECFP-C1-PHF13 (100-200)

Plasmid

#246112

Purpose

Expression of human PHF13 (100-200) fused to ECFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13(100-200) (PHF13 Human)

Tags

ECFP

Expression

Mammalian

Mutation

aa 101 - 200

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pECFP-C1-PHF13 (150-300)

Plasmid

#246111

Purpose

Expression of human PHF13 (150-300) fused to ECFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13(150-300) (PHF13 Human)

Tags

ECFP

Expression

Mammalian

Mutation

aa 151 - 300

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pECFP-C1-PHF13 (1-150)

Plasmid

#246110

Purpose

Expression of human PHF13 (1-150) fused to ECFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13(1-150) (PHF13 Human)

Tags

ECFP

Expression

Mammalian

Mutation

aa 1 - 150

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pECFP-C1-PHF13

Plasmid

#246107

Purpose

Expression of human PHF13 fused to ECFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Tags

ECFP

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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PEYFP-C1-PHF13∆PEST2

Plasmid

#246104

Purpose

Expression of human PHF13 with PEST2 deletion fused to mEYFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13∆PEST2 (PHF13 Human)

Tags

mEYFP

Expression

Mammalian

Mutation

del PEST2 ( del aa 141 - 189 )

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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