Addgene

We narrowed to 9,334 results for: FIE

Showing: 321 - 340 of 9334 results

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pLBT0040

Plasmid

#172800

Purpose

Level 1 module containing 35S-HSP21-nYFP_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-3XFLAGnYFP-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0060

Plasmid

#172802

Purpose

Level 1 module containing 35S-nYFP-HSP21_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-3XFLAGnYFP-mHSP21-CT_STOP-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0070

Plasmid

#172803

Purpose

Level 1 module containing 35S-cYFP-HSP21_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-cYFP3XHA-mHSP21-CT_STOP-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0080

Plasmid

#172804

Purpose

Level 1 module containing 35S-HSP21-nVENUS_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-nVENUS-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0090

Plasmid

#172805

Purpose

Level 1 module containing 35S-HSP21-cVENUS_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-3XFLAGcVENUS-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0100

Plasmid

#172806

Purpose

Level 1 module containing 35S-PTAC5-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mPTAC5-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0110

Plasmid

#172807

Purpose

Level 1 module containing 35S-deltaPTAC5-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-deltamPTAC5-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0020

Plasmid

#172776

Purpose

Level 0 module containing NT1_OEP7

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS of chloroplast outer envelope membrane protein OEP7

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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p-sfGFP-pA-FRT-Amp-FRT

Plasmid

#176620

Purpose

for generating PCR templates with sfGFP and Amp for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

super folder green fluorescent protein with FRT-flanked ampicillin cassette

Use

Unspecified

Tags

Expression

Mutation

H217R - please see dep. comments

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-Tev

Plasmid

#84555

Purpose

MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FKBP-20-Tev

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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TU#1412_tiam-1_cDNA

Plasmid

#82364

Purpose

express tiam-1 cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

tiam-1 cDNA (tiam-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

none

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1449_unc-73e_cDNA

Plasmid

#82363

Purpose

express unc-73e cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 e isoform (unc-73 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
pEThT-RvLEAM

Plasmid

#90033

Purpose

To express His-tagged RvLEAM in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Article

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T5/Lac

Available since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pPSup_35Senh(fwd)_35Spr+synJ

Plasmid

#149422

Purpose

Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Insert

35S core enhancer

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pZS*11_4enh

Plasmid

#149423

Purpose

Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Inserts

rbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer

Use

Tags

T120>A

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MNDU3

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBait

Plasmid

#165146

Purpose

Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSUP-(2xCouRS-tRNAopt)

Plasmid

#197930

Purpose

Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

Depositor

Kenneth Johnson

Article

Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

Inserts

CouRS
tRNA opt

Use

Tags

Expression

Bacterial

Mutation

Promoter

glnS' and lambda(ts) and proK

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBait-YY1

Plasmid

#165147

Purpose

Bait plasmid for PROBER with YY1-binding site (positive control)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

3X YY1 motif

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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