We narrowed to 2,344 results for: PUC19

1. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

   Plasmid

   #195505

   Purpose

   T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
   

   Use

   CRISPR; Donor vector

   Tags

   mRuby-3xFLAG-NLS-3xSV40-poly(A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

   Plasmid

   #121174

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

   Plasmid

   #121175

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. JM109

   Bacterial Strain

   #49761

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   None

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. JM106

   Bacterial Strain

   #49757

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pFloxed DHFR-TS*

   Plasmid

   #100605

   Purpose

   pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR

   Depositor

   David Sibley

   Insert

   Floxed TgDHFR-TS*
   

   Use

   Toxoplasma expression

   Tags

   Expression

   Mutation

   Pyrimethamine resistance

   Promoter

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. EasyClone-MarkerFree Vector Set

   Plasmid Kit

   #1000000098

   Purpose

   Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9

   Depositor

   Irina Borodina

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

8. JM107

   Bacterial Strain

   #49759

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. JM110

   Bacterial Strain

   #49763

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   Streptomycin

   Available since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pUC-ODN

    Plasmid

    #208642

    Purpose

    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Long single strand dna production

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR

    Plasmid

    #113252

    Purpose

    pUC19 backbone with E. coli CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    E. coli CRISPR array
    

    Use

    Integration substrate

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW091

    Plasmid

    #113253

    Purpose

    pUC19 backbone with S. pyogenes CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes CRISPR array
    

    Use

    Integration substrate

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-T7-J1~398-Luc

    Plasmid

    #208639

    Purpose

    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19

    Depositor

    Quanfu Ma

    Insert

    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    

    Use

    Luciferase; In vitro transcription template

    Tags

    Firefly Luciferase (F-Luc and T7 promoter

    Expression

    Mutation

    nt 1~398, in frame with donstream F-Luc

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT440

    Plasmid

    #8609

    Depositor

    Robert Hartley

    Article

    Yazynin et al Gene 1996 Feb 22;169(1):131-2.

    Insert

    barnase, barstar
    

    Use

    Tags

    as pMT416

    Expression

    Bacterial

    Mutation

    V36 codon of barnase of pMT416 replaced by polyli…

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pSelAct-KO

    Plasmid

    #174374

    Purpose

    Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

    Depositor

    Gitta Coaker

    Article

    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

    Type

    Empty backbone

    Use

    Vector for 2-step knockout by homologous recombin…

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSelAct-Express

    Plasmid

    #174375

    Purpose

    Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible

    Depositor

    Gitta Coaker

    Article

    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

    Type

    Empty backbone

    Use

    Expression vector for targeted integration

    Tags

    Expression

    Mutation

    Promoter

    pCMP1

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHB1

    Plasmid

    #204668

    Purpose

    pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

    Depositor

    Mark Mandel

    Article

    Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

    Insert

    FRT-erm-FRT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCVD432

    Plasmid

    #63755

    Purpose

    Probe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.

    Depositor

    James Kaper

    Article

    Baudry et al J Infect Dis. 1990 Jun;161(6):1249-51.

    Insert

    EAggEC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pILGFP1DFB

    Plasmid

    #185871

    Purpose

    A parental plasmid for in vivo gene amplification at RPL25 locus

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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