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Bacterial Strain#49763PurposeE.coli K-12 derivativeDepositorBacterial ResistanceStreptomycinAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only
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JM107
Bacterial Strain#49759PurposeE.coli K-12 derivativeDepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN
Plasmid#208642PurposeEmpty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.DepositorTypeEmpty backboneUseLong single strand dna productionTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#113252PurposepUC19 backbone with E. coli CRISPR arrayDepositorInsertE. coli CRISPR array
UseIntegration substrateTagsExpressionMutationPromoterNAAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW091
Plasmid#113253PurposepUC19 backbone with S. pyogenes CRISPR arrayDepositorInsertS. pyogenes CRISPR array
UseIntegration substrateTagsExpressionMutationPromoterNAAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-J1~398-Luc
Plasmid#208639Purposefor in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19DepositorInsertHepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
UseLuciferase; In vitro transcription templateTagsFirefly Luciferase (F-Luc and T7 promoterExpressionMutationnt 1~398, in frame with donstream F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT440
Plasmid#8609DepositorInsertbarnase, barstar
UseTagsas pMT416ExpressionBacterialMutationV36 codon of barnase of pMT416 replaced by polyli…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSelAct-KO
Plasmid#174374PurposePlasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatibleDepositorTypeEmpty backboneUseVector for 2-step knockout by homologous recombin…TagsExpressionMutationPromoterN/AAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSelAct-Express
Plasmid#174375PurposePlasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatibleDepositorTypeEmpty backboneUseExpression vector for targeted integrationTagsExpressionMutationPromoterpCMP1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHB1
Plasmid#204668PurposepUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructsDepositorInsertFRT-erm-FRT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVD432
Plasmid#63755PurposeProbe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.DepositorInsertEAggEC
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1DFB
Plasmid#185871PurposeA parental plasmid for in vivo gene amplification at RPL25 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterExpressionMutationUTR and partial CDS in-frame with F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT1004
Plasmid#8613DepositorInsertbinase, barstar
UseTagsExpressionBacterialMutationas pMT1002PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4BG6
Plasmid#185877PurposeA parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AA5
Plasmid#185875PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EC4
Plasmid#185880PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AG4
Plasmid#185874PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EF3
Plasmid#185881PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only