Addgene

We narrowed to 3,297 results for: aaas

Showing: 321 - 340 of 3297 results

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pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pPROEX Hsp104 (773-908)

Plasmid

#1316

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (773-908) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

deletion of aa1-773

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (361-548)

Plasmid

#1317

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (361-548) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa361-548 of Hsp104

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330) S278C

Plasmid

#87739

Purpose

Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-277, mutated Ser 278 to Cys

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(81-437)

Plasmid

#87734

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-80

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 F130W

Plasmid

#1302

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Phe 130 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 L553W

Plasmid

#1306

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 553 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pAM72

Plasmid

#227622

Purpose

pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Use

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and aa 2-239, G77P

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM71

Plasmid

#227621

Purpose

pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Use

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and amino acids 24-160-GGSGSG-192-221

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRS426-CeLACE-1

Plasmid

#174523

Purpose

Express C.elegans LACE-1 in yeast cells

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

lace-1 (C30F12.2 Nematode)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

MET25

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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MYMH 49

Plasmid

#138039

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-8AT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GTG AAA

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 56

Plasmid

#138042

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-9GA-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAA AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 53

Plasmid

#138041

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-9GT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAT AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 51

Plasmid

#138040

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-9GC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAC AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 47

Plasmid

#138038

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-8AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GCG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 45

Plasmid

#138037

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-8AG-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GGG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 43

Plasmid

#138036

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-7GA-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG AAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 40

Plasmid

#138034

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-7GC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG CAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 37

Plasmid

#138033

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-6GA-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAA GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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