We narrowed to 1,121 results for: gibson assembly

Showing: 321 - 340 of 1121 results

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pBTK130

Plasmid

#110584

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 promoter with lac operator and RBS

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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EZ-L498

Plasmid

#131771

Purpose

PPGK1_FUSN_Citrine _PixE_TCYC1

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PPGK1_FUSN_Citrine _PixE_TCYC1

Use

Integration into his3 locus

Expression

Yeast

Promoter

PGK

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-GEI-17(133-509)

Plasmid

#87056

Purpose

Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Publication

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)

Tags

6xHis-TEV and GAGGGS linker

Expression

Bacterial

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-T

Plasmid

#87522

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Publication

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-A

Plasmid

#87523

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Publication

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-C

Plasmid

#87524

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Publication

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-G

Plasmid

#87525

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Publication

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8-attR4-pegRNA-attR3

Plasmid

#213056

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attL4

Plasmid

#213055

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc12-HiBiT

Plasmid

#198308

Purpose

Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc12-HiBiT

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLXMA _RK2 L1A

Plasmid

#153348

Purpose

Expression vector for plants with low-copy number origin of replication

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary Δ

Plasmid

#153355

Purpose

Provides End-to-End linker 4Δ for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4Δ

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary Γ

Plasmid

#153354

Purpose

Provides End-to-End linker 4Γ for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4Γ

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary B

Plasmid

#153353

Purpose

Provides End-to-End linker 4B for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4B

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary 3

Plasmid

#153351

Purpose

Provides Middle-to-End linker 3 for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 3

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary 1

Plasmid

#153349

Purpose

Provides Middle-to-End linker 1 for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 1

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSIN-TRE-EB3N-VVDfast-mCl3-iLID

Plasmid

#190169

Purpose

Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-mClover3-iLID (MAPRE3 Synthetic, Human)

Use

Lentiviral

Tags

-VVDfast-mClover3-iLID

Mutation

1-189 aa from EB3

Promoter

TRE

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pL0_CD-greenlantern

Plasmid

#202141

Purpose

Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Green lantern gene

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-CEN_ARS_HIS

Plasmid

#202129

Purpose

Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CEN_ARS_HIS

Use

Synthetic Biology

Mutation

ARS sequence mutated to remove sapI site for dome…

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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