Addgene

We narrowed to 1,502 results for: naga

Showing: 321 - 340 of 1502 results

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Nano-lantern(Ca2+)_117/pRSETB

Plasmid

#51979

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2GS

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_150/pRSETB

Plasmid

#51978

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2G

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pattB-3xFlag-Loqs-PB

Plasmid

#41109

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PB (loqs Fly)

Use

Tags

3xFlag

Expression

Insect

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET15b+PCNA2 L171C

Plasmid

#66177

Purpose

Expresses the L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Use

Tags

His6 tag

Expression

Bacterial

Mutation

L171C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 G108C

Plasmid

#66140

Purpose

Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

G108C

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 WT

Plasmid

#66179

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 WT

Plasmid

#66175

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYI277

Plasmid

#228285

Purpose

Constitutive EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

KpGAPDH promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

Plasmid

#209089

Purpose

mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:cIPAD-3 (ΔICD)

Plasmid

#208518

Purpose

ddGFP-A and ddGFP-B3-inserted Pcdhα4 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:cIPAD-1 (ΔICD)

Plasmid

#208517

Purpose

ddGFP-A and ddGFP-B1-inserted Pcdhα4 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:B3-α4ΔICD

Plasmid

#208513

Purpose

ddGFP-B3-inserted Pcdhα4 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:B1-α4ΔICD

Plasmid

#208511

Purpose

ddGFP-B1-inserted Pcdhα4 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCX:α4ΔICD-EC4-GA

Plasmid

#208512

Purpose

ddGFP-A-inserted Pcdhα4 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_110μ-pcDNA3

Plasmid

#111929

Purpose

Cyan color bright luminescent calcium indicator with low affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Use

Tags

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+R2(L171C)

Plasmid

#108239

Purpose

Expresses PdR-PCNA2(L171C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C) fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

PCNA2,L171C

Promoter

T7 promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T+1X

Plasmid

#108188

Purpose

PCNA1-PdX fusion protein

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA1-PdX fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

C73S/C85S (PdX)

Promoter

T7 promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Use

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pPD1R

Plasmid

#86969

Purpose

Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH

Promoter

T7 promoter

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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p1G108CP20X

Plasmid

#85096

Purpose

Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The G108C variant of PCNA1 fused to putidaredoxin

Use

Tags

His6 tag

Expression

Bacterial

Mutation

G108C in PCNA1

Promoter

T7 promoter

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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