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  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University have developed...signal. Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored...imaging. Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing...
  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...
  3. Components of CRISPR/Cas9

    Type
    Blog Post
    ...NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola (TD) 3' NAAAAC  The...
  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...
  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean...polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-...Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring...
  6. Luciferase Plasmid Collection

    Type
    Collection
    ...CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...
  7. CRISPR Guide

    Type
    Collection
    ...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s...
  8. Chemogenetics Guide

    Type
    Guide
    ...eaav5282. PMID: 30872534 (Link opens in a new window) Nagai Y, Miyakawa N, Takuwa H, Hori Y, Oyama K, Ji B, ...
  9. Molecular Biology Reference

    Type
    Guide
    ...technology, which enables the preparation of more managable (i.e., smaller) DNA fragments. In 1952, Joshua...
  10. CRISPR Guide

    Type
    Guide
    ...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s...
Showing: 1 - 20 of 20 results