We narrowed to 21 results for: naga
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TypeBlog Post...luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University have developed...signal. Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored...imaging. Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing...
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Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent... -
Components of CRISPR/Cas9
TypeBlog Post...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC The... -
Fluorescent Protein Guide: Biosensors
TypeCollection...Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean...ACS Chem Biol. 2010 Feb 19;5(2):215-22. Takeharu Nagai ATP ATP sensor for Yeast (AT1.03 derived) Dynamic...polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-...Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock,... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post....2009.169 Dudek, A. M., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & ... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH... -
Luciferase Plasmid Collection
TypeCollection...CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression... -
CRISPR History and Development for Genome Engineering
TypeCollection...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...In order to overcome these limitations, Takeharu Nagai and colleagues at Osaka University have developed... -
CRISPR Guide
TypeCollection...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s... -
Chemogenetics Guide
TypeGuide...eaav5282. PMID: 30872534 (Link opens in a new window) Nagai Y, Miyakawa N, Takuwa H, Hori Y, Oyama K, Ji B, ... -
Adenovirus Guide
TypeGuide...) PMID: 17546019 (Link opens in a new window) Matsunaga, W., & Gotoh, A. (2023). Adenovirus as a vector... -
Molecular Biology Reference
TypeGuide...technology, which enables the preparation of more managable (i.e., smaller) DNA fragments. In 1952, Joshua...