We narrowed to 2,390 results for: puc19

1. pUC19-HDRT-CD3z-truncCAR(anti-CD19)

   Plasmid

   #215758

   Purpose

   HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2

   Depositor

   Dimitrios Wagner

   Article

   Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

   Insert

   CD247-truncCD19CAR (CD247, CD19 Human)
   

   Use

   Tags

   IgG1 CH3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTR-348 pUC19-tNGFR-P2A-ICOS N

   Plasmid

   #186066

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   ICOS (ICOS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTR-337 pUC19-tNGFR-P2A-CTLA4 N

   Plasmid

   #186054

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTR-342 pUC19-tNGFR-P2A-CD28 N

   Plasmid

   #186056

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CD28 (CD28 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

   Plasmid

   #110408

   Purpose

   Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

   Plasmid

   #89991

   Purpose

   Donor template for generation of SOX2-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

   Plasmid

   #174029

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-mScarlet
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

   Plasmid

   #89992

   Purpose

   Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

   Insert

   OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

   Plasmid

   #174028

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-eGFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid

    #169353

    Purpose

    Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

    Depositor

    Tony Carr

    Insert

    OsTIR1(F74A)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F74A

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid

    #208977

    Purpose

    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

    Plasmid

    #174026

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-QF2
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

    Plasmid

    #174030

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-Venus-PEST
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid

    #195505

    Purpose

    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    

    Use

    CRISPR; Donor vector

    Tags

    mRuby-3xFLAG-NLS-3xSV40-poly(A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid

    #121174

    Purpose

    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid

    #121175

    Purpose

    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT440

    Plasmid

    #8609

    Depositor

    Robert Hartley

    Article

    Yazynin et al Gene 1996 Feb 22;169(1):131-2.

    Insert

    barnase, barstar
    

    Use

    Tags

    as pMT416

    Expression

    Bacterial

    Mutation

    V36 codon of barnase of pMT416 replaced by polyli…

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pMT1004

    Plasmid

    #8613

    Depositor

    Robert Hartley

    Insert

    binase, barstar
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    as pMT1002

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI-VSVG

    Plasmid

    #1733

    Purpose

    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab

    Depositor

    Garry Nolan

    Insert

    VSV-G Envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC32 [TU#839]

    Plasmid

    #16079

    Depositor

    Martin Chalfie

    Insert

    mec-2 intron 9
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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