We narrowed to 3,199 results for: sam

1. pJL1 BSMT1

   Plasmid

   #106287

   Purpose

   Expresses BSMT1 in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   BSMT1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAct:dCas9-VP64

   Plasmid

   #78901

   Purpose

   Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC_C_FGFR2

   Plasmid

   #187751

   Purpose

   MAC-tagged gene expression of human FGFR2

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   FGFR2 (FGFR2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAct:MS2-p65-HSF

   Plasmid

   #78902

   Purpose

   Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   MS2-p65-HSF
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

   Plasmid

   #180267

   Purpose

   Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   EGFR sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

   Plasmid

   #180273

   Purpose

   Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

   Plasmid

   #180266

   Purpose

   Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   No-Target Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SuExp His-Myc-mPLD3

   Plasmid

   #173853

   Purpose

   Protein expression plasmid for recombinant His-Myc tag mouse PLD3

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD3 (Pld3 Mouse)
   

   Tags

   6xHis, Myc, Factor X cleavable

   Expression

   Mammalian

   Mutation

   intracellular and transmembrane domain truncated,…

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC3-FGFR1

    Plasmid

    #222611

    Purpose

    Expression of MAC3-tagged FGFR1

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    FGFR1 (FGFR1 Human)
    

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180268

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    GARP sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG MCP-VP64

    Plasmid

    #92357

    Purpose

    CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    MCP-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. MoFGFR2b-ires neo

    Plasmid

    #86491

    Purpose

    mamalian cell expression of FGFR2b

    Depositor

    David Ornitz

    Article

    Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

    Insert

    FgfrR2 IIIb 13A4 (FGFR2 Human)
    

    Expression

    Mammalian

    Promoter

    Moloney murine leukemia virus LTR

    Available Since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TAL2317

    Plasmid

    #36743

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FGFR1-TALEN_Right (FGFR1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TAL2316

    Plasmid

    #36742

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FGFR1-TALEN_Left (FGFR1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CBh-mKate2-IRES-MCS (WT-WT) (AAV2)

    Viral Prep

    #105921-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-CBh-mKate2-IRES-MCS (WT-WT) (#105921). In addition to the viral particles, you will also receive purified pAAV-CBh-mKate2-IRES-MCS (WT-WT) plasmid DNA. Control mKate2 expression AAV with two wild-type ITR sequences. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marcella Patrick

    Promoter

    CBh and same CBh promoter as mKate2

    Tags

    mKate2

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

17. FGFR1-optoDroplet

    Plasmid

    #111509

    Purpose

    For optogenetic clustering of FGFR1

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2, FUS, Fusion Red, and Myristoylation sequenc…

    Promoter

    SFFV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SuExp His-Myc-mPLD4

    Plasmid

    #201185

    Purpose

    Protein expression plasmid for recombinant His-Myc tag mouse PLD4

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD4 (Pld4, Pld4, Pld4 Mouse)
    

    Tags

    His-Myc, Factor Xa cleavable

    Expression

    Mammalian

    Mutation

    cytosolic and transmembrane domain truncated; add…

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG150 FGFR1 3'UTR

    Plasmid

    #12008

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N15 3'UTR (FGFR1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. psiCHECK GSC 3'UTR

    Plasmid

    #19859

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    GSC 3'UTR (GSC Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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