We narrowed to 4,339 results for: Pet2;

1. pET28-sfGFP-SpyTag003 Loop B

   Plasmid

   #171777

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-SpyTag003 Loop B
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   SpyTag003 and linkers inserted between residues A…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-sfGFP-SpyTag003 Loop C

   Plasmid

   #171778

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-SpyTag003 Loop C
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   SpyTag003 and linkers inserted between residues A…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-anticalin (E143amb)-His

   Plasmid

   #168046

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Glu 143 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-anticalin (S87amb)-His

   Plasmid

   #168044

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ser 87 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-anticalin (N116amb)-His

   Plasmid

   #168045

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 116 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-anticalin (N21amb)-His

   Plasmid

   #168041

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 21 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-anticalin (I55amb)-His

   Plasmid

   #168042

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ile 55 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pet22b-mEGFP-YAP-C-IDR

   Plasmid

   #166441

   Purpose

   Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAP-C-IDR(266-504) (YAP1 Human)
   

   Expression

   Bacterial

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

   Plasmid

   #136688

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression w/ three mut…

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET24a-VHH-tev-2xTS

    Plasmid

    #117750

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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