We narrowed to 15,064 results for: pET

1. Methyl-binding domain protein 2 (MBD2) coiled-coil domain

   Plasmid

   #132721

   Purpose

   Bacterial expression vector for methyl-binding domain protein 2 (MBD2) coiled-coil domain for use as competitor peptide in measurements of effective concentrations

   Depositor

   Magnus Kjaergaard

   Article

   Sorensen et al Methods Mol Biol. 2020;2141:505-518. doi

   Insert

   Methyl-binding protein domain 2 coiled-coil domain
   

   Use

   Tags

   N-terminal His-tag. Flanking linkers to match acc…

   Expression

   Bacterial

   Mutation

   Promoter

   T/

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SIN-GluR1-AcGFPnuc-WPRE

   Plasmid

   #245092

   Purpose

   Lentiviral vector with nuclear fluorescent reporter AcGFP expressed from Rat GluR1 promoter

   Depositor

   Nicole Déglon

   Article

   Delzor et al Hum Gene Ther Methods. 2012 Aug;23(4):24

   Insert

   AcGFP
   

   Use

   Lentiviral

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Rat GluR1 promoter

   Available Since

   Oct. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBD-PYR1-MANDI

   Plasmid

   #241279

   Purpose

   Yeast two hybrid vector expressing BD-PYR1-MANDI

   Depositor

   Sean Cutler

   Article

   Park et al Nature. 2015 Apr 23;520(7548):545-8. doi

   Insert

   PYR1_mandi (PYR1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX458-SLC35A1

   Plasmid

   #239952

   Purpose

   Encodes gRNA for human SLC35A1

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   SLC35A1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX458-MFSD6

   Plasmid

   #239951

   Purpose

   Encodes gRNA for human MFSD6

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   MFSD6
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. M6667

   Plasmid

   #219772

   Purpose

   MoClo-compatible Level 0 vector carrying terminator ORCA3

   Depositor

   Karen Sarkisyan

   Article

   Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

   Insert

   Terminator ORCA3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. M6570

   Plasmid

   #219773

   Purpose

   MoClo-compatible Level 0 vector carrying promoter pORCA3

   Depositor

   Karen Sarkisyan

   Article

   Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

   Insert

   Promoter ORCA3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pORCA3

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNK3625

   Plasmid

   #219770

   Purpose

   MoClo-compatible Level 0 vector carrying promoter pWRKY70

   Depositor

   Karen Sarkisyan

   Article

   Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

   Insert

   Promoter WRKY70
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pWRKY70

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. M6597

   Plasmid

   #219771

   Purpose

   MoClo-compatible Level 0 vector carrying terminator WRKY70

   Depositor

   Karen Sarkisyan

   Article

   Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

   Insert

   Terminator WRKY70
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-EFS-MCP-3XBFP-2xNLS

    Plasmid

    #236371

    Purpose

    Overexpression of MCP-3XBFP-2xNLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    MCP-3XBFP-2xnls
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE-EFS-MCP-3XBFP-NLS

    Plasmid

    #236372

    Purpose

    Overexpression of MCP-3XBFPnls in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    MCP-3XBFPnls
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiCRISPRv2-ICAM-5-Puro

    Plasmid

    #239949

    Purpose

    CRISPR KO of human ICAM-5

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    ICAM-5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiCRISPRv2-MFSD6-Puro

    Plasmid

    #239950

    Purpose

    CRISPR KO of human MFSD6

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD6
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE-EFS-Halo-NLS-tDeg

    Plasmid

    #236370

    Purpose

    Overexpression of Halo-NLS-tDeg in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    Halo-NLS-tDeg
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FHpHG_mTent5c_dCAT

    Plasmid

    #221442

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5c_AA (dCAT) (Fam46c Mouse)
    

    Use

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Mutation

    D90A, D92A (GATctgGAT to GCCctgGCC)

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FHpHG_mTent5a

    Plasmid

    #221439

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5a (Fam46a Mouse)
    

    Use

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FHpHG_mTent5c

    Plasmid

    #221440

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5c (Fam46c Mouse)
    

    Use

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH

    Plasmid

    #235277

    Purpose

    ComMAND closed-loop circuit regulating mRuby2 with miR-FF5

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a-mGL-bGH

    Plasmid

    #235299

    Purpose

    mGreenLantern expression to serve as a transfection marker, EF1a-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH

    Plasmid

    #235282

    Purpose

    ComMAND open-loop circuit regulating mRuby2 with miR-FF4

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH

    Plasmid

    #235260

    Purpose

    ComMAND open-loop circuit regulating mRuby2 with miRE-FF5

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EFS-mGL-bGH

    Plasmid

    #235255

    Purpose

    mGreenLantern expression to serve as a transfection marker, EFS-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS (human EF1a short, intronless)

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hPGK-mRuby2-bGH

    Plasmid

    #235254

    Purpose

    ComMAND base gene regulating mRuby2, hPGK-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK (human phosphoglycerate kinase)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EF1a-mRuby2(miR.FF5)-TS.FF6x1-bGH

    Plasmid

    #235276

    Purpose

    ComMAND open-loop circuit regulating mRuby2 with miR-FF5

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH

    Plasmid

    #235270

    Purpose

    ComMAND open-loop circuit regulating mRuby2, EF1a

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH

    Plasmid

    #235271

    Purpose

    ComMAND closed-loop circuit regulating mRuby2, EF1a

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH

    Plasmid

    #235283

    Purpose

    ComMAND closed-loop circuit regulating mRuby2 with miR-FF4

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EF1a-TS.FF4x1-mRuby2-bGH

    Plasmid

    #235274

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH

    Plasmid

    #235261

    Purpose

    ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EF1a-tagBFP-bGH

    Plasmid

    #235257

    Purpose

    tagBFP expression to serve as a "filler" plasmid for transfections

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    tagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to remove BsaI sites in tagBFP

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EF1a-mGL(miRE.FF4)-bGH

    Plasmid

    #235264

    Purpose

    microRNA expression for dual-vector iFFL circuit

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EFS-mRuby2-bGH

    Plasmid

    #235253

    Purpose

    ComMAND base gene regulating mRuby2, EFS-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS (human EF1a short, intronless)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EF1a-mRuby2-bGH

    Plasmid

    #235269

    Purpose

    ComMAND base gene regulating mRuby2, EF1a

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EF1a-TS.FF6x1-mRuby2-bGH

    Plasmid

    #235275

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EF1a-mRuby2-TS.FF4x1-bGH

    Plasmid

    #235272

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EF1a-mRuby2-TS.FF6x1-bGH

    Plasmid

    #235273

    Purpose

    3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAG415Gal_Gcn4 SATtoG KtoRHA

    Plasmid

    #232994

    Purpose

    Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG415Gal_Gcn4 SATtoG KtoR

    Plasmid

    #232961

    Purpose

    Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAG415Gal_Gcn4 ILVtoED solvvol

    Plasmid

    #232958

    Purpose

    Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED solvvol
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG415Gal_Gcn4 SATtoG KtoRGFP

    Plasmid

    #233006

    Purpose

    Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pssAAV_Seq17_CMV_bglobin_3NLS_RFP_WPRE_polyA

    Plasmid

    #220222

    Purpose

    AAV-STARR-seq validation vector with RFP

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pssAAV_Seq17_CMV_bglobin_3NLS_GFP_WPRE_polyA

    Plasmid

    #220221

    Purpose

    AAV-STARR-seq validation vector with GFP

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDest17_Gcn4 ILVtoED solvvol

    Plasmid

    #231858

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED solvvol
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    T7

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCIFR-8

    Plasmid

    #227666

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    AmR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFNC-8

    Plasmid

    #227670

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. AmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    AmR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR

    Plasmid

    #203963

    Purpose

    Dox inducible myc tag truncated SOX9 without trans-activating domain (noTA)

    Depositor

    Elaine Fuchs

    Article

    Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

    Insert

    SOX9-noTA (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mutation

    the TA domain is truncated

    Promoter

    TRE3G

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR

    Plasmid

    #203964

    Purpose

    Dox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)

    Depositor

    Elaine Fuchs

    Article

    Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

    Insert

    SOX9-noHMG (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mutation

    the HMG domain is truncated

    Promoter

    TRE3G

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. SBC015898

    Plasmid

    #226285

    Purpose

    Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

    Depositor

    Rainer Breitling

    Article

    Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    Eugenol O-methyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAM221

    Plasmid

    #226645

    Purpose

    pET24b-p97-R191Q-His6

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R191Q

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAM224

    Plasmid

    #226647

    Purpose

    pET24b-p97-T262A-His6

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    T262A

    Promoter

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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