We narrowed to 8,070 results for: Pet2

1. pET28a- mCherry-FIVAR-HIS-ybbR

   Plasmid

   #169138

   Purpose

   E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   mCherry
   

   Tags

   6x Histag, FIVAR tag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SdrG-B1-ELP-HIS-ybbR

   Plasmid

   #169133

   Purpose

   E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21b-Cdc42/13C-FKBP-MBP

   Plasmid

   #214291

   Purpose

   Express split-Cdc42 fragment fused with FKBP and MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Split-Cdc42 fragment fused with FKBP (CDC42 Human)
   

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Cdc42-Q61L

   Promoter

   T7

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b-Rac1/13C-FKBP-MBP

   Plasmid

   #214295

   Purpose

   Express split-Rac1 fragment fused with FKBP and MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Split-Rac1 fragment fused with FKBP (RAC1 Human)
   

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Rac1-Q61L

   Promoter

   T7

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21b-RhoA/13C-FKBP-MBP

   Plasmid

   #214299

   Purpose

   Express split-RhoA fragment fused with FKBP and MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Split-RhoA fragment fused with FKBP (RHOA Human)
   

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   RhoA-Q63L

   Promoter

   T7

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b-KRas/13C-FKBP-MBP

   Plasmid

   #214303

   Purpose

   Express split-KRas fragment fused with FKBP and MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Split-KRas fragment fused with FKBP (KRAS Human)
   

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   KRas-Q61L

   Promoter

   T7

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-GAL4-PARP1-CAT-L713F

   Plasmid

   #175949

   Purpose

   Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   GAL4 DNA binding domain (GAL4 Budding Yeast)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   L713F, V762A for the 'PARP1-CAT-L713F' …

   Promoter

   T7

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b-effector-ncRNA-rrt-His8

   Plasmid

   #194089

   Purpose

   Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 effector-ncRNA-rrt
   

   Tags

   His8

   Promoter

   T7

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-H12TEV-hEIF5B-587~1220

   Plasmid

   #208637

   Purpose

   for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220

   Depositor

   Quanfu Ma

   Insert

   human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   contain SNP rs1376443, protein sequence not chang…

   Promoter

   T7

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid

    #208628

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 1~41, other residues not included

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_mouse-Pro-IL-18_humanized-triple-mutant

    Plasmid

    #214339

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (Il18 Mouse)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    ENGD29DDEN_N36Y_H41E

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET24a-His6-CUbo(K63R)-MCS

    Plasmid

    #212727

    Purpose

    Bacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET24a-His6-CUbo(K48R)-MCS

    Plasmid

    #212726

    Purpose

    Bacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

    Plasmid

    #208630

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    Δ1~34,Δ215~284

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

    Plasmid

    #208632

    Purpose

    for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 53~77, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

    Plasmid

    #208624

    Purpose

    for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 1~77, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-His8-aCat(660-865)

    Plasmid

    #203335

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-865 only

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-His8-aCat(660-885)

    Plasmid

    #203334

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-885 only

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET24a His6 GST PYL2(14-188)

    Plasmid

    #177842

    Purpose

    Bacterial Expression of PYL2

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    PYR1-like 2 (PYL2 Mustard Weed)
    

    Tags

    6xHis-GST

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-Fgβ-anticalin-His-ybbr

    Plasmid

    #168040

    Purpose

    Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Tags

    6x Histag, Fgβ peptide, and ybbr tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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