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We narrowed to 6,251 results for: cas9 expression plasmid

Showing: 3401 - 3420 of 6251 results

  1. pCRISPEY-Z3-Hyg

    Plasmid
    #232101
    Purpose
    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232101-plasmid-map-sequence-id-462305

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  5. pJM108

    Plasmid
    #134160
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    KMLL, E. coli codon optimized, CRISPR/Cas9 harden…
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134160-plasmid-map-sequence-id-271380

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  6. pJM107

    Plasmid
    #134159
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134159-plasmid-map-sequence-id-271374

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  8. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101725-plasmid-map-sequence-id-199888

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  10. PEA1-Nuclease-Puro

    Plasmid
    #171992
    Purpose
    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171992-plasmid-map-sequence-id-338139

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  11. PEA1-Nuclease-GFP

    Plasmid
    #171994
    Purpose
    Delivers all prime editing nuclease components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171994-plasmid-map-sequence-id-338231

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  12. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  13. pGLOW39

    Plasmid
    #173068
    Purpose
    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms
    Depositor
    Ryan Doonan
    Article
    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791
    Insert
    mScarlet-I-C1^SEC^3xMyc
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173068-plasmid-map-sequence-id-345944

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  17. AAV-TRE-scFVDnmt3A_bGHpA

    Plasmid
    #177355
    Purpose
    AAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177355-plasmid-map-sequence-id-353667

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  18. AAV-TRE-scFVDnmt3L_bGHpA

    Plasmid
    #177356
    Purpose
    AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    194–415 aa
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177356-plasmid-map-sequence-id-353665

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  19. AAV-TRE-scFVtet1_bGHpA

    Plasmid
    #177357
    Purpose
    AAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    N terminal domain of human Tet1
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177357-plasmid-map-sequence-id-377038

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  20. AAV-TRE-scFVtet2_bGHpA

    Plasmid
    #177358
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177358-plasmid-map-sequence-id-353663

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Showing: 3401 - 3420 of 6251 results