We narrowed to 17,901 results for: REV

1. AFG3-2EGFP-ter-URA3

   Plasmid

   #184770

   Purpose

   Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   AFG3 (AFG3 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SYS1-2EGFP-ter-URA3

   Plasmid

   #184771

   Purpose

   Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   SYS1 (SYS1 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET23PI-Sce ESARC D218A

   Plasmid

   #195608

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T928C, T929C, A942C

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET23PI-Sce ESARC

   Plasmid

   #195607

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

   Promoter

   T7

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NAT-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193314

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Use

   Tags

   4MYC

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193315

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Use

   Tags

   4FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HYG-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193316

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Use

   Tags

   4MYC

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SEC7-2Katushka-ter-Hygromycin

   Plasmid

   #184772

   Purpose

   Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   SEC7 (SEC7 Budding Yeast)
   

   Use

   Tags

   2Katushka

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PKC1-2GFP-ter-TRP1

   Plasmid

   #184773

   Purpose

   Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   PKC1 (PKC1 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXD70LacZ4-PdegV-LacZ

    Plasmid

    #191620

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoter

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXD70LacZ8.1-Pcadh-LacZ

    Plasmid

    #191624

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pXD70LacZ9-Phcdh-LacZ

    Plasmid

    #191625

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXD70Tet(DadRmt1)-DadR(ΔDBD)

    Plasmid

    #191637

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ΔDBD

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXD70Tet(DadRmt2)-DadR(ΔTM)

    Plasmid

    #191638

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ΔTM

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXD70Tet(HcdR)-HcdR

    Plasmid

    #191648

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    HcdR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXD70Tet(CadR)-CadR

    Plasmid

    #191649

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), cadR complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. eSpCas9-EF

    Plasmid

    #177267

    Purpose

    CRISPR vector

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1-mScarlet-AMPKα2

    Plasmid

    #192451

    Purpose

    mScarlet-tagged alpha2 isoform of AMPK catalytic subunit.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    mScarlet-AMPKalpha2 (Prkaa2 Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-EF1a-GFP RanSen

    Plasmid

    #184697

    Purpose

    Lentiviral transfer plasmid for expression of GFP RanSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP RanSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-EF1a-ArgSen (-)NLS

    Plasmid

    #184689

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKLV2-EF1a-ArgSen

    Plasmid

    #184691

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-EF1a-ArgSen

    Plasmid

    #184700

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYI047

    Plasmid

    #170843

    Purpose

    (pFRO6::SMT2-FLAG::tOCS)

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    pFRO6::SMT2-FLAG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    FRO6p

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYI048

    Plasmid

    #170844

    Purpose

    (pGH3.17::SMT2-FLAG::tOCS)

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    pGH3.17::SMT2-FLAG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GH3.17p

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYI012

    Plasmid

    #170841

    Purpose

    pGH3.17::GUSPlus::tOCS

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    GH3.17p
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GH3.17p

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FnRPR9-10

    Plasmid

    #182568

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FnRPR8-10

    Plasmid

    #182570

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBXPHM3-NB-D6

    Plasmid

    #167989

    Purpose

    expression of a nanobody against rat PepT2 as a n terminal MBP fusion

    Depositor

    Simon Newstead

    Article

    Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

    Insert

    NanobodyD6
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

    Plasmid

    #186274

    Purpose

    Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas6-RT-Cas1
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. DpbCas12e

    Plasmid

    #188495

    Purpose

    Expresses FLAG tagged DpbCas12e

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PlmCas12e

    Plasmid

    #188496

    Purpose

    Expresses FLAG tagged PlmCas12e

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. DpbdCas12e KRAB

    Plasmid

    #188505

    Purpose

    Expresses FLAG tagged DpbdCas12e KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D672A/E769A/D935A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Cas12j3

    Plasmid

    #188497

    Purpose

    Expresses FLAG tagged Cas12j3

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPpT4- HTA1prom-TIR1-FLAG

    Plasmid

    #189725

    Purpose

    Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    HTA1

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPpT4- TEF2prom-TIR1-FLAG

    Plasmid

    #189726

    Purpose

    Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pILGFP4D42

    Plasmid

    #185840

    Purpose

    Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pIALD2E

    Plasmid

    #185866

    Purpose

    Knocking out ALD6

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD(-125, 40)- ALD6(1054, 1749)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pILGFP10CF71

    Plasmid

    #185860

    Purpose

    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pILGFP10CF72 (B9)

    Plasmid

    #185858

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pILGFP10CF72

    Plasmid

    #185857

    Purpose

    Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pILGFP10E1B

    Plasmid

    #185856

    Purpose

    Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pILGFP10E1A

    Plasmid

    #185855

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pILGFP3A2 (1)

    Plasmid

    #185851

    Purpose

    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCfB9358(VII-1_Markerfree_BackBone)

    Plasmid

    #161599

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCfB9359(VIII-1_Markerfree_BackBone)

    Plasmid

    #161600

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCfB9361(XIII-1_Markerfree_BackBone)

    Plasmid

    #161602

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCfB9363(XVI-1_Markerfree_BackBone)

    Plasmid

    #161604

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. KBB439

    Plasmid

    #185093

    Purpose

    NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    GAL1::NMA111-GFP

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. KBB462

    Plasmid

    #185095

    Purpose

    NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    GAL1::nma111Dnls1Dnls2-GFP

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge