We narrowed to 18,569 results for: MUT

1. pCas9CR4-VQR

   Plasmid

   #120224

   Purpose

   pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

   Depositor

   Christopher Reisch

   Insert

   SpCas9-VQR
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Mutation

   Mutations D1135V/R1335V/T1337R

   Promoter

   pTet

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCCL-c-MNDU3-CD3D c.202C>T-cDNA

   Plasmid

   #198022

   Purpose

   LV Expression of CD3D cDNA with mutation at c.202C>T used as a disease editing target for CD3d SCID mutaiton identified in Mennonite population

   Depositor

   Donald Kohn

   Insert

   CD3D c.202C>T-cDNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Codon optimized (20 bp) on either end of cDNA for…

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-Ex1-17-deltaP1P2-PTPN22

   Plasmid

   #195391

   Purpose

   Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 and P2 domains) with FLAG tag

   Depositor

   Patrick Concannon

   Insert

   PTPN22 (PTPN22 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   PTPN22 mutant containing exon 1 to 17 and lacking…

   Available Since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-Ex1-17-deltaP2-PTPN22

   Plasmid

   #195395

   Purpose

   Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P2 domain) with FLAG tag

   Depositor

   Patrick Concannon

   Insert

   PTPN22 (PTPN22 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   PTPN22 mutant containing exon 1 to 17 and lacking…

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-Ex1-17-deltaP1-PTPN22

   Plasmid

   #195388

   Purpose

   Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 domain) with FLAG tag

   Depositor

   Patrick Concannon

   Insert

   PTPN22 (PTPN22 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   PTPN22 mutant containing exon 1 to 17 and lacking…

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEYFP-N1-HsSmg5-siRNAres_I

   Plasmid

   #146541

   Purpose

   Mammalian Expression of HsSmg5-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   HsSmg5-siRNAres (SMG5 Human)
   

   Expression

   Mammalian

   Mutation

   three non silent mutation A254G (K85R), T980C (L3…

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIneo-lambdaN-HA-HsSmg5_I

   Plasmid

   #146531

   Purpose

   Mammalian Expression of HsSmg5

   Depositor

   Elisa Izaurralde

   Insert

   HsSmg5 (SMG5 Human)
   

   Expression

   Mammalian

   Mutation

   three non silent mutation A254G (K85R), T980C (L3…

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIneo-lambdaN-HA-HsSmg7-siRNAres_I

   Plasmid

   #146536

   Purpose

   Mammalian Expression of HsSmg7-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   HsSmg7-siRNAres (SMG7 Human)
   

   Expression

   Mammalian

   Mutation

   three silent mutation G216A, T1185C and A1521G an…

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRMCD77

   Plasmid

   #173032

   Purpose

   Modified pET17b (Novagen) vector

   Depositor

   Renato Morona

   Article

   Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

    Plasmid

    #146166

    Purpose

    Insect Expression of DmUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF2-siRNAres (Upf2 Fly)
    

    Expression

    Insect

    Mutation

    3 non silent mutations: L1022P, V1097L and N1213S…

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK573

    Plasmid

    #72456

    Purpose

    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    synthetic gene with ~57 silent substitutions

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK541

    Plasmid

    #72447

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK569

    Plasmid

    #72455

    Purpose

    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    synthetic gene with ~51 silent substitutions

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK021

    Plasmid

    #75060

    Purpose

    Produces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    E. coli optimized codons in first 25 residues

    Promoter

    lac

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIB184-Km

    Plasmid

    #90195

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

    Type

    Empty backbone

    Tags

    None

    Expression

    Bacterial

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLZts

    Plasmid

    #128799

    Purpose

    Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

    Depositor

    Timothy Barnett

    Article

    Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

    Type

    Empty backbone

    Use

    Bacterial mutagenesis

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHyo245

    Plasmid

    #173147

    Purpose

    Expresses pheS_A294G by dual T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pheS_A294G
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHyo182

    Plasmid

    #173136

    Purpose

    Expresses pheS_A294G in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pheS_A294G
    

    Expression

    Bacterial

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3-hPXR-FL-T248E

    Plasmid

    #41617

    Depositor

    Sridhar Mani

    Insert

    hPXR-FL-T248E (NR1I2 Human)
    

    Expression

    Mammalian

    Mutation

    N10D and T248E

    Promoter

    CMV

    Available Since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCDNA3-hPXR-FL-K277Q

    Plasmid

    #41618

    Depositor

    Sridhar Mani

    Insert

    hPXR-FL-K277Q (NR1I2 Human)
    

    Expression

    Mammalian

    Mutation

    N10D and K277Q

    Promoter

    CMV

    Available Since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only