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We narrowed to 402 results for: MUT

Showing: 1 - 20 of 402 results

  1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ... single mutagenic primer pair to generate an individual mutant, this technique uses mutagenic primers ...2nd mutant strand: The second mutant strand is synthesized in the same manner as the first mutant strand...Pooled Libraries to Generate Mutant Populations Use REPLACER Mutagenesis to Mutate Your Plasmids Resources...libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation mutagenesis as a...generate complex libraries of mutant plasmids ready for deep mutational scanning.     3 reasons to be...to one pot saturation mutagenesis Several methods can be used to create mutational scanning libraries. ...favoring of certain types of mutations, difficulty controlling the rate of mutagenesis, and low throughput. ...

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...directed mutagenesis to generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your... to mutagenesis with error prone PCR, EvolvR uses an error prone polymerase to introduce mutations. However... 15-20 bp. EvolvR’s on-target mutagenesis rate was 2.5 x 10-6 mutations per nucleotide per generation ...increase the mutation rate and extend the editing window of EvolvR: (1) two additional mutations to PolI3M... PolI5M which increased EvolvR’s mutagenesis rate to ~10-3 mutations per nucleotide per generation, and...many known spectinomycin resistance mutations, several new mutations were identified by targeting EvolvR...Mutagenesis is a tool that both evolution and molecular biologists use to tinker with DNA. Making changes...

  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... the mutagenesis reaction. Alternative approaches to site directed mutagenesis Point-mutagenesis is fairly...designed mutations (not limited to frame shift mutations). Consequently, site directed mutagenesis is one...contains the template, site-directed mutagenesis can be used to mutate the PAM sequence (an NGG sequence ...cleavage. Summary of Site Directed Mutagenesis In brief, point-mutations can be introduced to plasmids using...  Site-directed mutagenesis of Plasmids. A) Generation of mutagenized plasmids. PCR primers (green...through mutagenesis vastly facilitates the subsequent process of screening for succesfully mutated clones...site directed mutagenesis.  Polymerase To ensure that you don’t introduce undesired mutations through the...

  4. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ... point mutations Insertions, deletions, point mutations Insertions, deletions, point mutations Insertions...inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM can help determine...directed mutagenesis methods Current SDM methods rely on in vitro assembly. To make a point mutation, you ...desired mutation, then use those to PCR amplify the template plasmid. The parental, nonmutated DNA is ...REPLACR-mutagenesis is that you must PCR amplify the whole plasmid, which could introduce mutations. Using...bp homology, the observed efficiency of mutagenesis (mutation-containing colonies/total colonies) is about...REPLACR-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works ...

  5. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...attempting to characterize mutations in a pooled cell population, as mutations with strong phenotypic effects...types of mutations they identified included gene amplifications and fusions, missense mutations, and indels...Functional Annotation of Somatic Mutations in Cancer.” Of all the mutations and fusions represented in this...their associated mutants in other model systems. It is possible that a given mutation will have a different...Thousands of mutations have been implicated in cancer. Yet, the majority of them remain uncharacterized...first functional characterization of over 1,000 mutations, and the group has deposited several hundred plasmids...assortment of genes. Using patient data to identify mutations of unknown importance in cancer Previously available...

  6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...characterized by deficient phosphomannomutase (PMM) activity due to mutations in the PMM2 gene. Videira...diseases. Spontaneous mutations are a major cause of rare diseases. Spontaneous mutations that change protein...diseases caused by Arg-mutant proteins, but there is currently no evidence of Arg-mutant protein rescue in ...rescue multiple Arg-mutant proteins in living baker’s yeast cells, including Arg-mutant yeast homologs of...research on diseases of glycosylation, arginine mutagenesis, and neural development. Paula Videira: Congenital...27393411. Michael McMurray: Rescuing arginine mutations in rare disease with guanidine Michael McMurray...other. Indeed, nearly 20% of the single missense mutant alleles that cause human disease change Arg to ...

  7. Biosensor AAV Preps

    Type
    Collection
    ...Constitutive 9 Li 140555 pAAV-hsyn-GRAB_DA-mut Syn GRAB_DA-mut (control) none Constitutive 9 Li 140556 pAAV-hsyn-GRAB_rDA1m...Constitutive 9 Li 140558 pAAV-hsyn-GRAB_rDA-mut Syn GRAB_rDA-mut (control) none Constitutive 9 Li 208702 ...Constitutive 9, rg* Li 123310 pAAV-hSyn-GRAB_NEmut Syn GRAB_NEmut (control) none Constitutive 9, rg* Li ...Constitutive 1, 9 Li 185388 pAAV-hSyn-GRAB_OTmut Syn GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage...

  8. Brain Initiative Collection

    Type
    Collection
    ...AAV9 pAAV-hsyn-GRAB_DA-mut Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons Yulong Li ...pAAV-hsyn-GRAB_rDA-mut Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons Yulong...NE) control sensor GRAB_NEmut in neurons Yulong Li 123310-AAVrg pAAV-hSyn-GRAB_NEmut Expresses the genetically-encoded...GRAB_NE1h in neurons Yulong Li 123310-AAV9 pAAV-hSyn-GRAB_NEmut Expresses the genetically-encoded fluorescent...fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons Yulong Li 125560-AAV1 pAAV-CAG-dLight1.3b...Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana...

  9. Open Enzyme Collection

    Type
    Collection
    ...Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase... Transcriptase (RNAse H deactivated by 3 mutations) 165556 pOpen-MMLV_RT (lack H) Moloney Murine Leukemia...

  10. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...mismatch/mutation may be the result of a mis-called peak in the trace file. If the mutation is not an...such as the gene/insert, fusion proteins, point mutations, deletions, etc.) involves selecting one or more...Addgene sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence...

  12. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...use a high fidelity taq polymerase to minimize mutations. The fidelity of the polymerase becomes more important...PCR based cloning carries a much higher risk for mutation than restriction enzyme based cloning. DNA replication...

  13. Genetic Code Expansion

    Type
    Collection
    ...changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church " 68306 C321.ΔA... UAG codons changed to UAA. mutS+, λ-, ΔybhB-bioAB, ΔprfA, ΔserB mutations for improved yield and growth...endogenous TAG codons mutated to TAA, RF1 (prfA) deleted, fabR spontaneously mutated, serB deleted for phosphoserine...jannaschii , M. barkeri , or E.coli and can be mutated and screened through directed evolution to charge.... mazei Mammalian TAG Ivana Nikić-Spiegel 182881 Mut1-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine...propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram 182882 Mut2-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine...

  14. Gibson Assembly Protocol

    Type
    Protocol
    ...making a small change in a plasmid (such as point mutations). Generate DNA segments by PCR. Run PCR product...

  17. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...blocking mutation. Despite the fact that both the blocking mutation and our intended mutation were on ...or heterozygous mutations. 1. Increasing HDR accuracy with CRISPR/Cas-blocking mutations After genome editing...sequence. The idea was that these “blocking mutations” mutations prevent CRISPR from re-cutting the target...blocking mutations? This depends entirely on the locus you are editing, and if the blocking mutation needs...intended mutation while taking advantage of the improved efficiency provided by blocking mutations. Essentially...cut site to mutation distance and editing efficiency.   3. Optimizing "cut-to-mutation distance" for... Alzheimer mutations we study are heterozygous in patients. As the probability of mutation incorporation...

  18. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...Surveyor® or T7E1) for mutation detection. While these methods paved the way for mutation validation, we found...and is not capable of binding a mutated target. The amount of both mutated and wild type target in each ...samples with mono-allelic mutations versus those with the bi-allelic mutations typically desired when screening... comes time to (hopefully) validate successful mutation of your favourite gene, there are several different... to help guide you through the rough waters of mutation validation, such as this "CRISPR 101" post. Next-generation... molecule in a sample. Digital PCR assays for mutation screening Now that we have introduced how digital... assays can be used to sniff out your desired mutations. The assays you will use aren’t that much different...

  19. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... don’t have a specific mutation in mind. Generating a random library of mutants using error-prone PCR ...employed to mutate whole antibody sequences, degenerate primers can be designed to selectively mutate specific...can shrink your mutant library and increase the chances of finding beneficial mutations. In other words...control and efficiency! Screening mutant libraries After generating your mutant libraries, you have to screen...more, the best mutant can then be used as the starting point in the next round of mutant library generation...typically come in when generating the library of mutants and how they are screened. When conducting antibody...When you complete each cycle, your best-performing mutant is usually the starting point for the next cycle...

  20. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...uptake, iron uptake receptor mutation. T1/T5 Phage resistance gal Mutation in galactose metabolism pathway...DNA, preparing unmethylated DNA, and more. Many mutations that make these features possible are present ...present in most commercial strains, especially mutations that make major improvements such as those that increase...found in E. coli strains. Table 1: Common gene mutations found in E.coli strains Gene(s) Description...Functional Consequence dam DNA adenine methylase mutation (GATC) Preparing unmethylated DNA, important when...ex: ClaI or XbaI) dcm DNA cytosine methylase mutation (CCWGG) Preparing unmethylated DNA, important ...enzymes that are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of...
Showing: 1 - 20 of 402 results