We narrowed to 24,891 results for: Spr

1. pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS

   Plasmid

   #244837

   Purpose

   Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   LbCas12a-RVQ
   

   Use

   CRISPR

   Tags

   2xSV40 NLS

   Expression

   Mammalian

   Mutation

   G146R/R182V/E795Q

   Promoter

   T7

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP1020

   Plasmid

   #239942

   Purpose

   AtACT2 promoter driving the expression of dCas9-ZAT10(2x)

   Depositor

   Ryan Lister

   Article

   Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

   Insert

   AtACT2::dCas9-ZAT10(2x)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Mutation

   N/A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP1011

   Plasmid

   #239933

   Purpose

   dCas9 without Stop codon as Level-0 part (Golden Gate cloning)

   Depositor

   Ryan Lister

   Article

   Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP1010

   Plasmid

   #239932

   Purpose

   dCas9 with Stop codon as Level-0 part (Golden Gate cloning)

   Depositor

   Ryan Lister

   Article

   Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK660

   Plasmid

   #242914

   Purpose

   Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

   Insert

   PspF-λN22 (pspF Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   BBa_J23107

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS641_Flex-Cas12a-ABE

   Plasmid

   #244846

   Purpose

   Mammalian expression of Flex-Cas12a adenine base editor

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Flex-dCas12a-ABE
   

   Use

   CRISPR

   Tags

   SV40 NLS-ABE8e

   Expression

   Mammalian

   Mutation

   G146R/R182V/D535G/S551F/D665N/E795Q/D832A

   Promoter

   CMV

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMKR07

   Plasmid

   #240097

   Purpose

   A CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native host

   Depositor

   Adrian Keatinge-Clay

   Article

   Wang et al Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   ermE* promoter

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT16

   Plasmid

   #223388

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT15

   Plasmid

   #223387

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT17

    Plasmid

    #223389

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQAT26

    Plasmid

    #223398

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQAT28

    Plasmid

    #223400

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQAT29

    Plasmid

    #223401

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQAT30

    Plasmid

    #223402

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQAT14

    Plasmid

    #223386

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9-EcRT-Z3-Kan

    Plasmid

    #232096

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Z3

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEL1027

    Plasmid

    #225598

    Purpose

    The STU backbone vector for rice genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEL1028

    Plasmid

    #225599

    Purpose

    The STU backbone vector for maize genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRS-AraC-dCas9

    Plasmid

    #220195

    Purpose

    mini-Tn7 dCas9 expression vector - AraC/pBAD promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    AraC/pBAD

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRhaCAST

    Plasmid

    #211791

    Purpose

    pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter

    Depositor

    Silvia Cardona

    Article

    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Rhamnose-inducible

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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