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pcDNA-DEST47-SIRT5

Plasmid

#242135

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT5 (SIRT5 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST47-SIRT6

Plasmid

#242136

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT6 (SIRT6 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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AAVTRE3_smFP_HA

Plasmid

#201209

Purpose

AAV vector for TRE(TRE3G)-driven smFP_HA (HA-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _HA (addgene plasmid #59759)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_163

Plasmid

#210774

Purpose

C1_Porcine techovirus-1 P2A skipping peptide (P2A)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

P2A Skipping peptide

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-MET25pr

Plasmid

#221100

Purpose

Methionine-repressible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-MET25pr

Plasmid

#221103

Purpose

Methionine-repressible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-CUP1pr

Plasmid

#221106

Purpose

Copper-inducible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxNeon

Plasmid

#229942

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxNeon

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMS13

Plasmid

#229474

Purpose

Expresses RssB with Q254A & N256A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A, N256A)

Expression

Bacterial

Mutation

Q254A, N256A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF53

Plasmid

#229477

Purpose

expresses RssBE229C

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E229C)

Expression

Bacterial

Mutation

E229C

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBD1

Plasmid

#229463

Purpose

Expresses RssB

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Expression

Bacterial

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF30

Plasmid

#229472

Purpose

Expresses RssB with R115A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (R115A)

Expression

Bacterial

Mutation

R115A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF29

Plasmid

#229473

Purpose

Expresses RssB with N256A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (N256A)

Expression

Bacterial

Mutation

N256A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF28

Plasmid

#229469

Purpose

Expresses RssB with D104A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF31

Plasmid

#229468

Purpose

Expresses RssB with Q254A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A)

Expression

Bacterial

Mutation

Q254A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF42

Plasmid

#229467

Purpose

Expresses RssB with S29C & E229C mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (S29C, E229C)

Expression

Bacterial

Mutation

S29C, E229C

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF16

Plasmid

#229465

Purpose

Expresses RssB with D58P mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D58P)

Expression

Bacterial

Mutation

D58P

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS26

Plasmid

#229471

Purpose

Expresses RssB with D104N mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104N)

Tags

10xHis

Expression

Bacterial

Mutation

D104N

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS23

Plasmid

#229470

Purpose

Expresses RssB with D104A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Tags

10xHis

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS11

Plasmid

#229464

Purpose

Expresses RssB with His Tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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