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Plasmid#147347PurposeInsect Expression of DmeIF1ADepositorInsertDmeIF1A (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeEF1A_R
Plasmid#147349PurposeInsect Expression of DmeIF1ADepositorInsertDmeIF1A (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen
Plasmid#184691PurposeLentiviral transfer plasmid for expression of ArgSenDepositorInsertArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-ArgSen
Plasmid#184700PurposeLentiviral transfer plasmid for expression of ArgSenDepositorInsertArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MAPK9-P2A-Hygro_Barcode
Plasmid#170238PurposeBarcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAPK9 (MAPK9 Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2592.EF1a.SLC6A1.S295L.RFP.P2A.PURO.JL.p163
Plasmid#170174PurposeLentiviral Expression of EF1a.SLC6A1.S295L.RFPDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationS295LPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2592.EF1a.SLC6A1.A288V.P2A.PURO.JL.p166
Plasmid#170171PurposeLentiviral Expression of EF1a.SLC6A1.A288VDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationA288VPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-HaloSFPQY527A
Plasmid#166949PurposeLentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoterDepositorInsertSFPQ-Y527A (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationSFPQ-Y527APromotereF1aAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-EGFPKir7.1
Plasmid#107181PurposeLentiviral particle generationDepositorInsertKCNJ13 (KCNJ13 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterEF1AAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Ccdc167
Plasmid#120242PurposeExpresses V5-tagged Ccdc167 in lentiviral vectorDepositorInsertCcdc167 (Ccdc167 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Tmem128
Plasmid#120239PurposeExpresses V5-tagged Tmem128 in lentiviral vectorDepositorInsertTmem128 (Tmem128 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti U6-sgRNA/EF1a-mCherry
Plasmid#114199PurposeLentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoterDepositorInsertsgRNA scaffold
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863APromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.BB.3z
Plasmid#200679PurposeModular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXR001: EF1a-CasRx-2A-EGFP
Plasmid#109049PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targetingDepositorInsertCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationPromoterEF1AAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro
Plasmid#85132Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-VEGFA EF1a-rtTA3
Plasmid#195881PurposeLentiviral construct of doxycycline inducible VEGFADepositorInsertVEGFA (Vegfa Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_EF1a-DjCas13d-msfGFP-Blast
Plasmid#226008PurposeDjCas13d protein for RNA targeting in piggyBac backboneDepositorInsertDjCas13d RNA-targeting nuclease
UseCRISPRTagsmsfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC407 - pAAV EF1a eGFP
Plasmid#60058PurposeAn AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.DepositorInsertenhanced Green Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-H2B-miRFP670
Plasmid#212668PurposeExpress tagged H2BDepositorInsertH2B (H2BC11 Human)
UseLentiviralTagsmiRFP670ExpressionMutationPromoterEF-1aAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-EF1A>Tet3G:IRES:Neo
Plasmid#198756PurposeVector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418DepositorInsertTet3G, IRES, Neo
UseLentiviralTagsExpressionMutationPromoterEF1AAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEnt L1L3 EF1a-tTA-2
Plasmid#26803DepositorInsertElongation Factor 1 alpha promoter and tTA transgene
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Blast
Plasmid#85133Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-Cre
Plasmid#121675PurposeExpresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)DepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9InsertNLS-CRE-HA
UseAAVTags3xHA and SV40-NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-JRGECO1a
Plasmid#128317PurposeExpresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.DepositorHas ServiceAAV1InsertNES-JRGECO1a
UseAAVTagsHis TagExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-MCP-huADARE488QT490A
Plasmid#159922PurposeFusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5DepositorInsertHuman ADAR2 E488QT490A (ADARB1 Human)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-eFFly-mCherry
Plasmid#104833Purpose3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detectionDepositorInsertseFFly
mCherry
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas3
Plasmid#178881PurposeExpression of human codon-optimized NlaCas3 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas3c-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP
Plasmid#55641PurposeFlp-Dependent EYFPDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6s
Plasmid#105714PurposeCan be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.DepositorHas ServiceAAV8InsertGCaMP6s
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterEf1a, Human elongation factor-1 alphaAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only