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Plasmid#61573PurposeTarget a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 geneDepositorInsertRasgrf2-2A-dCre (Rasgrf2 Mouse, P1 bacteriophage)
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1,NR
Plasmid#218395Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuccessor
Plasmid#228391Purposeintended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithographyDepositorInsertpConst23100-sfGFP__pLambda-DHFR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB228i16
Plasmid#209738PurposeColA BBa_B0014 tetAp SD8 G1_PylRS-[mCNPR43] T3Te-T7Te DHFRDepositorInsertG1PylRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB228i24
Plasmid#209739PurposeColA BBa_B0014 tetAp SD8 G1_PylRS-[pCNPR02] T3Te-T7Te DHFRDepositorInsertG1PylRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB228r3
Plasmid#209741PurposeColA BBa_B0014 tetAp SD8 Lum1PylRS-[PylHRS*] T3Te-T7Te DHFRDepositorInsertMlum1RS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB228p
Plasmid#209742PurposeColA BBa_B0014 tetAp SD8 MmPylRS-[wt] T3Te-T7Te DHFRDepositorInsertMmPylRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB228p4
Plasmid#209743PurposeColA BBa_B0014 tetAp SD8 MmPylRS-[AF] T3Te-T7Te DHFRDepositorInsertMmPylRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
UPR pBAL Ura3
Plasmid#45353DepositorInsertDHFR Ub Lek Ura3
UseTagsExpressionYeastMutationPromoterpCUP1Available sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDownstream1Lox
Plasmid#164574PurposeIntegration of 1 LoxN site in the genome of P. bergheiDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB228q12
Plasmid#209740PurposeColA BBa_B0014 tetAp SD8 MjTyrRS-[p-TpaRS-1*] T3Te-T7Te DHFRDepositorInsertMjTyrRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB228v
Plasmid#209744PurposeColA BBa_B0014 tetAp SD8 ScTrpRS-[5OH-R3-13] T3Te-T7Te DHFRDepositorInsertScTrpRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB228v6
Plasmid#209745PurposeColA BBa_B0014 tetAp SD8 ScTrpRS-[ScTrpRS-H15] T3Te-T7Te DHFRDepositorInsertScTrpRS
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAH4
Plasmid#41828PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistanceDepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGJJ001
Plasmid#183619PurposebindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp
Plasmid#211788PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpRDepositorInsertdhfr
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAH2
Plasmid#41826PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7, TEFAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSJ95
Plasmid#104343PurposeExpress yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertFBP1
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSJ125
Plasmid#104344PurposeExpress yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertMDH2
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAH3
Plasmid#41827PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only