Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation.
Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, Atsuta-Tsunoda K, Aoki K, Tsukiji S
Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
172100pCMV-EGFP-eDHFR(69K6)-MCSMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)
172101pCMV-MCS-eDHFR(69K6)-EGFPMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP
172102pPBpuro-mNG-eDHFR(69K6)-Tiam1Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)
172103pPBpuro-mNG-eDHFR(69K6)-p85iSH2Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)
172104pCSIIpuro-mNG-eDHFR(69K6)-RasGEFMammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)
172105pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-sMammalian expression of Gαs fused to miRFP670-eDHFR(69K6)
172106pCAGGS-mNG-eDHFR(69K6)-G-alpha-qMammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)
172107pPBpuro-cRaf-mNG-eDHFR(69K6)Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)
178849pPBpuro-EGFP-eDHFR(69K6)-cRafMammalian expression of cRaf fused to EGFP-eDHFR(69K6)
178850pPBpuro-EGFP-eDHFR(69K6)Mammalian expression of EGFP-eDHFR(69K6)
178851pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)
178852pCSIIpuro-miRFP670-eDHFR(69K6)Mammalian expression of miRFP670-eDHFR(69K6)
178853pCSIIpuro-mNG-eDHFR(69K6)Mammalian expression of mNeonGreen-eDHFR(69K6)
178854pCAGGS-chGrb2/eDHFR(69K6)-iRFP713Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713
178855pCAGGS-eDHFR(69K6)-iRFP713Mammalian expression of eDHFR(69K6)-iRFP713
178856pCAGGS-PKCdelta-EGFPMammalian expression of PKCδ fused to EGFP
178857pCAGGS-chPKCdelta/eDHFR(69K6)-EGFPMammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP
178858pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670
178859pCMV-miRFP670-eDHFR(69K6)Mammalian expression of miRFP670-eDHFR(69K6)
178860pCAGGS-EGFP-SOS1Mammalian expression of SOS1 fused to EGFP

Antibodies from Article