We narrowed to 2,255 results for: LOB;

1. pEHISSSO1986

   Plasmid

   #97057

   Purpose

   Expressing Sulfolobus solfataricus orf SSO1986 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   S. solfataricus orf SSO1986
   

   Use

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEAHISSO1404

   Plasmid

   #97056

   Purpose

   Expressing Sulfolobus solfataricus orf SSO1404 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   S. solfataricus orf SSO1404
   

   Use

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEAHISSOarD1

   Plasmid

   #97067

   Purpose

   Expressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   S. solfataricus Sso Ard1 protein
   

   Use

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEAHISSIRV131

   Plasmid

   #97053

   Purpose

   Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   SIRV orf 131
   

   Use

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEAHISSIRV418/gp25

   Plasmid

   #97052

   Purpose

   Expressing Sulfolobus islandicus rudivirus (SIRV) orf 418/gp25 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   SIRV orf 418/gp25
   

   Use

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXT7

   Plasmid

   #32995

   Depositor

   Sergei Sokol

   Type

   Empty backbone

   Use

   Xenopus rna expression

   Tags

   globin 3'UTR and globin 5' UTR

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-CS-AfPiwi_G

   Plasmid

   #146383

   Purpose

   Bacterial Expression of AfPiwi

   Depositor

   Elisa Izaurralde

   Insert

   AfPiwi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   2 non silent mutations A350G (E117G) and A872G (R…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pInt_attP1_kanR

   Plasmid

   #205294

   Purpose

   Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pInt_attP2_kanR

   Plasmid

   #205297

   Purpose

   Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#2).

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pInt_attP3_chlorR

    Plasmid

    #205298

    Purpose

    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#3).

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pInt_attP5_ampR

    Plasmid

    #205299

    Purpose

    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#5).

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pInt_attP1_chlorR

    Plasmid

    #205295

    Purpose

    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pInt_attP1_ampR

    Plasmid

    #205296

    Purpose

    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pInt_attP1_sacB_kanR

    Plasmid

    #205301

    Purpose

    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Sperm whale Mb 64Y68F in pVP80K

    Plasmid

    #86956

    Purpose

    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

    Depositor

    John Olson

    Insert

    Mb 64Y68F
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    64Y68F

    Promoter

    T5

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInt_attP1_LCS_kanR

    Plasmid

    #205300

    Purpose

    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pInt_attP1_tsXis_sacB_kanR

    Plasmid

    #205302

    Purpose

    Integrating plasmid for ORBIT in E. coli with self excising factor (Xis) and sacB for counter selection. Used for markerless modifications.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKP515

    Plasmid

    #159597

    Purpose

    Express E.coli FepA protein

    Depositor

    Kathleen Postle

    Article

    Vakharia et al J Bacteriol. 2002 Oct;184(19):5508-12. d

    Insert

    fepA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

    Plasmid

    #203349

    Purpose

    Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

    Depositor

    Christopher Grigsby, Molly Stevens

    Insert

    mGreenLantern
    

    Use

    Template for t7-mediated in vitro transcription o…

    Tags

    Flanked by 5' and 3' UTRs from Xenopus …

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHelper_NoMutL_V2_gentR

    Plasmid

    #205293

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge