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We narrowed to 165 results for: LOB;

Showing: 1 - 20 of 165 results

  1. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...improving professional development and training in a globalized world where individuals can exchange ideas, network... of that, in the midst of a rapidly changing, globalized environment re-emerging from international financial... mentorship programs like IMP build bridges and global networks. They catalyze the development of knowledge...integrative and eco-social health strategy, and global health equity. She is also Co-founder/director ...

  3. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...kDa MWCO column, Millipore Sigma UFC903024 50 mL LoBind tubes, VWR 76289-498 Before Starting Wipe down ...collection tube each time. Place column in a new 50 mL LoBind collection tube. Slowly apply the sample to the...Gently transfer the sample from the reservoir to a LoBind tube. Pro-Tip Do not scrape the filter with the...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...IGKJ1 immunoglobulin kappa joining 1 J1 IGKJ2 immunoglobulin kappa joining 2 J2 IGKJ3 immunoglobulin kappa...IGKJ4 immunoglobulin kappa joining 4 J4 IGKJ5 immunoglobulin kappa joining 5 J5 IGKV@ immunoglobulin kappa...CD79a molecule, immunoglobulin-associated alpha IGA, MB-1 CD79B CD79b molecule, immunoglobulin-associated ...MGC102857 IGHA2 immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant...MGC29633 IGHD@ immunoglobulin heavy diversity group IGD1, IGHDY1 IGHD1-1 immunoglobulin heavy diversity...IGHD1-14 immunoglobulin heavy diversity 1-14 (non-functional) DM2, IGHD114 IGHD1-20 immunoglobulin heavy ... IGHD120 IGHD1-26 immunoglobulin heavy diversity 1-26 IGHD126 IGHD1-7 immunoglobulin heavy diversity 1...

  5. Gibson Assembly Protocol

    Type
    Protocol
    ...assembly of DNA molecules up to several hundred kilobases. Nature Methods , 6(5), 343–345. https://doi.org...

  7. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...AiE0086m-minRho-SYFP2-WPRE3-BGHpA AiP11580 AiE0086m SYFP2 Globus pallidus pars externa (GPe) Pallidum 230807 pAAV-hsA2...-minRho-iCre(R297T)-BGHpA AiP20171 AiE0086m Cre Globus pallidus pars externa (GPe) Pallidum 233570 pAAV-AiE0027m...SYFP2 Astrocytes Whole Brain 208133 pAAV-AiE0387m-minBglobin-SYFP2-WPRE3-BGHpA AiP12102 AiE0387m SYFP2 Astrocytes...Endothelial cells Whole Brain 208146 pAAV-AiE0395h-minBglobin-SYFP2-WPRE3-BGHpA AiP12157 AiE0395h SYFP2 Oligodendrocytes...Oligodendrocytes Whole Brain 208150 pAAV-AiE0397m-minBglobin-SYFP2-WPRE3-BGHpA AiP12123 AiE0397m SYFP2 Oligodendrocytes...Oligodendrocytes Whole Brain 208157 pAAV-AiE0410m-minBglobin-SYFP2-WPRE3-BGHpA AiP12109 AiE0410m SYFP2 Oligodendrocytes...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...both DNA strands via the NUC lobe, as in Figure 6. NUC lobe, green; REC lobe, light blue; guide RNA, dark... has two major lobes, called NUC (for nuclease) and REC (for recognition). The NUC lobe contains the DNA... primarily by the REC lobe. An arginine-rich bridge helix connects the two lobes and helps recognize the...Summary of Cas9 domains, the lobe each domain belongs to, and their roles. Lobe Domain Role NUC... yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue.   The REC lobe binds tightly...the REC lobe, stabilizing the closed conformation, as in Figure 3. Upon DNA binding, the NUC lobe recognizes...Figure 1: A cartoon depiction of Cas9’s two major lobes, REC and NUC, and their subdomains. NUC includes...

  9. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...to the patients. For example, defects in hemoglobin (𝛽-globin), which cause sickle cell disease and 𝛽...region of gamma hemoglobin (HGB1/2) leads to continuous expression of fetal hemoglobin even in adult patients...edited the fetal hemoglobin promoter and caused persistent expression of fetal hemoglobin to a greater level...can be mitigated with the expression of fetal hemoglobin. Previous studies have shown that one base change...

  10. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells for ... repression in yeast Base editing CRISPRi in Caulobacter crescentus Multiplexed gRNA for human pluripotent...dCas9. Image adapted from Guzzo et al., 2020. Caulobacter crescentus is a popular model organism for studying...system to downregulate expression of specific Caulobacter genes using a catalytically dead Cas9 (dCas9)...Streptococcus pyogenes system does not work well in Caulobacter). To test their CRISPRi system, the Laub lab ...

  11. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ... find themselves on the front lines of emerging global challenges. However, these same researchers face...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution...this map and increase plasmid sharing across the globe! We hope that these awards will both accelerate ...developing countries. As the world continues to face global challenges, we hope to empower those that need ...Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check out the List of Institutions...

  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...response, the World Health Organization has declared a global health emergency and called for the fast-tracked...emerging outbreak, as many as 2.2 billion people globally live in areas conducive to the spread of the virus...combined biomolecular tools can distinguish between global strains of the Zika virus (Asian-, African- and...that we can get the diagnostics into the hands of global health responders as soon as possible. Our longer-term...PMC4243060. 4. Messina, Jane P., et al. "Mapping global environmental suitability for Zika virus." Elife...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Immunoprecipitation (IP) uses immobilized antibodies, or immunoglobulins, to isolate a specific protein out of a complex...proteins bind only to the Fc portion of the immunoglobulin, leaving the antigen binding sites free to ...Protein A and Protein G have an affinity for immunoglobulins, the strength of the interaction varies between...will bind both the primary antibody and serum immunoglobulins indiscriminately, causing competition for binding...its use as an immunosorbent for isolation of immunoglobulins. FEBS Letters 28:73–76 Additional resources...

  14. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...used mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes...termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought to be more efficient in terminating...of AAUAAA are required for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131...transcription Polyadenylation: General overview BGH rbGlob SV40 Resources on the Addgene Blog Learn how ...

  15. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...increase accessibility to researchers around the globe. Addgene’s educational resources As Addgene built...designed and easy-to-reference eBooks. Figure 3: Global readership of the Addgene blog in 2023. Views from...videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel, 2023...available and accessible to scientists across the globe?” By asking this question, and by listening to the...

  16. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Neisseria meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...).  At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even smaller than SaCas9 ...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...

  18. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...than APC. While APC's global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum ...however, excites at 561 nm, which is close to the global maximum of PE's excitation spectrum where the excitation...maxima, one local maximum at just under 500 nm and a global maximum at around 560 nm, leading to different ...

  19. Microbiology Resources

    Type
    Collection
    ...maripaludis Methanothermobacter thermautotrophicus Sulfolobus islandicus Plasmids for Bacteria Species Anabaena...Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus...

  20. AAV Packaged on Request

    Type
    Collection
    ...reproducible for scientists everywhere and help build a globally connected scientific community. The Data Hub captures... measures. We have distributed preps around the globe and shared our well-developed and trusted protocols...
Showing: 1 - 20 of 165 results