Affinity Purification of Recombinant Antibodies with Protein A or Protein G
Type
Protocol
...kDa MWCO column, Millipore Sigma UFC903024 50 mL LoBind tubes, VWR 76289-498 Before Starting Wipe down ...collection tube each time. Place column in a new 50 mL LoBind collection tube. Slowly apply the sample to the...Gently transfer the sample from the reservoir to a LoBind tube. Pro-Tip Do not scrape the filter with the...