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pASK-mSAN1-Streptag

Plasmid

#117165

Purpose

Expresses Strep-tagged murine SAN1 nuclease in bacteria

Depositor

Ian Macara

Publication

Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

Insert

mSAN1 (Fam120b Mouse)

Tags

Streptag

Expression

Bacterial

Promoter

tetR/tetA

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-VPH2 chr I

Plasmid

#41897

Purpose

Yeast expression of VPH2.

Depositor

Dan Gottschling

Publication

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VPH2 (VPH2 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available since

Feb. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pGL4.27_GRHL-SPE

Plasmid

#177774

Purpose

Luciferase reporter for GRHL

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GRHL

Use

Luciferase

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYAB

Plasmid

#217670

Purpose

For expression of SUMO tagged human alpha B crystallin

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYAB (CRYAB Human)

Tags

6XHis and Smt3

Expression

Bacterial

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSH1

Plasmid

#90295

Purpose

Expresses the fat-7 gene in C. elegans intestine

Depositor

Anne Brunet

Publication

Han et al Nature. 2017 Apr 13;544(7649):185-190. d

Insert

fat-7 (fat-7 Nematode)

Tags

No

Expression

Worm

Promoter

ges-1

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-B

Plasmid

#177785

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYBA3

Plasmid

#80747

Purpose

Expression of human beta A3-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBA3 (CRYBA1 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Publication

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pTORGm43GWY

Plasmid

#112906

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Publication

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GW

Plasmid

#112902

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.

Depositor

Sebastian Schornack

Publication

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWR

Plasmid

#112905

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Publication

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSH9

Plasmid

#90328

Purpose

Expresses the set-2.a cDNA isoform in the C. elegans intestine

Depositor

Anne Brunet

Publication

Han et al Nature. 2017 Apr 13;544(7649):185-190. d

Insert

Pges-1::set-2a

Expression

Worm

Promoter

ges-1

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Publication

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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p416TEF-NYFP

Plasmid

#99553

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Publication

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWC

Plasmid

#112903

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Publication

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTORGm43GWF

Plasmid

#112904

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Publication

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTL-4

Plasmid

#52033

Purpose

Inducible expression of GST-unc43 in E. coli

Depositor

Meng-Qiu Dong

Publication

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

Unc-43 (unc-43 Nematode)

Tags

GST

Expression

Bacterial

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pICSL86977OD:PITG_15278a

Plasmid

#173545

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Publication

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_15278_P17777

Expression

Plant

Promoter

35S

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_14371

Plasmid

#173567

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Publication

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_14371_T30-4

Expression

Plant

Promoter

35S

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_11947

Plasmid

#173566

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Publication

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_11947_T30-4

Expression

Plant

Promoter

35S

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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