We narrowed to 3,304 results for: golden gate

1. pICH47732:FCP:NAT

   Plasmid

   #85984

   Purpose

   Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   NAT
   

   Use

   Golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   FCP

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGE114

   Plasmid

   #120731

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolytica

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Type

   Empty backbone

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNLS-LEXY (pDN138)

   Plasmid

   #72657

   Purpose

   golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Type

   Empty backbone

   Use

   Golden gate entry vector

   Tags

   NLS and mCherry-LEXY

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-GG-gRNA

   Plasmid

   #226860

   Purpose

   Human gRNA expression vector backbone with BsmBI Golden Gate site

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTB3-BC2 nanobody

   Plasmid

   #210208

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   BC2 Nanobody
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH71431

   Plasmid

   #50344

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. LI C-D GUS

   Plasmid

   #104517

   Purpose

   LI Gene of Interest module

   Depositor

   Thomas Lahaye

   Article

   Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

   Insert

   UidA gene
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICSL80005

   Plasmid

   #50322

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GGE146

   Plasmid

   #120742

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_4UASxpr-TEF)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P1_4UASxpr-TEF
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICH41421

    Plasmid

    #50339

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICH41414

    Plasmid

    #50337

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-Kan_US71_APikL1

    Plasmid

    #123646

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    US71_APikL1
    

    Use

    Golden gate

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL12006

    Plasmid

    #50270

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter + 5'UTR (Cassava Vein Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pICSL30008

    Plasmid

    #50300

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    N terminal HA tag (6x Human influenza hemagglutinin)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LI F-G Spec

    Plasmid

    #104521

    Purpose

    LI resistance gene module

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Spectinomycin resistance gene
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL30009

    Plasmid

    #50301

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    N terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GGE367

    Plasmid

    #120736

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Antibiotic resistance marker for selection after transformation in Y. lipolytica (Marker_hph)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    Marker_hph
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GGE151

    Plasmid

    #120751

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_4UASxpr-TEF)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P2_4UASxpr-TEF
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICSL50009

    Plasmid

    #50309

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal HA tag (6x Human influenza hemagglutinin)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICSL50004

    Plasmid

    #50316

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mCherry variant of RFP (Discosoma sp.)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge