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pCMK105

Plasmid

#226805

Purpose

Integrates KanR into glpK, 570 bp homology, three homologous HIP1 sites in left homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK74

Plasmid

#226657

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, KanR, contains HIP1 site

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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GH-t2a GFP

Plasmid

#194883

Purpose

an overexpression vector of the killifish growth hormone

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

growth hormone

Use

Lentiviral

Tags

t2a-GFP

Promoter

CMV

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLSPR22

Plasmid

#217307

Purpose

Promoterless (SwaI Cutsite) amdS:mRuby3 fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Tags

mRuby3

Expression

Yeast

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLSPR23

Plasmid

#217308

Purpose

Promoterless (SwaI Cutsite) amdS:mNeonGreen fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Tags

mNeonGreen

Expression

Yeast

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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FSHB-GFP fused

Plasmid

#205596

Purpose

an overexpression vector of the killifish FSHB fused to GFP

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

FSHB

Tags

GFP

Promoter

CMV

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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FSHB-t2a-dTOMATO

Plasmid

#205597

Purpose

an overexpression vector of the killifish FSHB

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

FSHB

Tags

dTOMATO

Promoter

CMV

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-SpGalpha-i

Plasmid

#185568

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

Galpha-i

Use

In vitro transcription (mrna synthesis)

Tags

GFP, S-Tag

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1L-GFP

Plasmid

#185574

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1L

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a_SUS1 5'SS

Plasmid

#100991

Purpose

Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

GGTAtGT->tGTAtGa 5'SS mutant

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a_No_BP-pUC18

Plasmid

#100992

Purpose

Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

TACTAAC->GTTAGTG BP mutataion

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc19-RBS21

Plasmid

#171885

Purpose

Promoter SPLc19 and RBSc21 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc19 and RBSc21

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc19-RBSc249

Plasmid

#171904

Purpose

Promoter SPLc19 and RBSc249 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc19 and RBSc249

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc25-RBSc123

Plasmid

#171900

Purpose

Promoter SPLc25 and RBSc123 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc25 and RBSc123

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc45-RBSc21

Plasmid

#171887

Purpose

Promoter SPLc45 and RBSc21 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc45 and RBSc21

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc45-RBSc123

Plasmid

#171901

Purpose

Promoter SPLc45 and RBSc123 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc45 and RBSc123

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc45-RBSc110

Plasmid

#171896

Purpose

Promoter SPLc45 and RBSc110 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc45 and RBSc110

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc3-RBSc110

Plasmid

#171893

Purpose

Promoter SPLc3 and RBSc110 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc3 and RBSc110

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc25-RBSc48

Plasmid

#171890

Purpose

Promoter SPLc25 and RBSc48 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc25 and RBSc48

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc47-RBSc249

Plasmid

#171907

Purpose

Promoter SPLc47 and RBSc249 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc47 and RBSc249

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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