We narrowed to 8,513 results for: sgrna

1. pRW006-SM-destination-pro35S-Cas9

   Plasmid

   #104439

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   35S

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

   Plasmid

   #207760

   Purpose

   MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pMXS-IRES-Blast SHMT2res

   Plasmid

   #106301

   Purpose

   Expresses SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 2 (SHMT2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations destroy sgRNA targeting sites

   Promoter

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAWp92

   Plasmid

   #157975

   Purpose

   pBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI}

   Depositor

   Alan Siu Lun Wong

   Article

   Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPN007

   Plasmid

   #91575

   Purpose

   Express sgRNA targeting human BCL11A

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   BCL11A (BCL11A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Sha2Cas9-HF

   Plasmid

   #192142

   Purpose

   Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   Sha2Cas9-HF
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Sha2Cas9-HF (R247A and N415A)

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti_dNmeCas9

   Plasmid

   #120077

   Purpose

   dNmeCas9 with no sgRNA cassette lenti construct

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   dNmeCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LRG-2.1T-neo-sgTAF12(human)#4.4

   Plasmid

   #105590

   Purpose

   lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   gRNA targeting human TAF12 HFD
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LCV2 V-1

   Plasmid

   #84804

   Purpose

   Cas9 with sgRNA targeting Exon 1 of VEGF-A

   Depositor

   Glenn Yiu

   Article

   Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

   Insert

   gRNA targeting VEGF-A (VEGFA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAWp100

    Plasmid

    #157977

    Purpose

    pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPN098

    Plasmid

    #91625

    Purpose

    Express sgRNA targeting human HCN1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    HCN1 (HCN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPN099

    Plasmid

    #91626

    Purpose

    Express sgRNA targeting human HCN1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    HCN1 (HCN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCR1272

    Plasmid

    #111107

    Purpose

    Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCI gRNA (FANCI Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCR1162

    Plasmid

    #111098

    Purpose

    Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCA gRNA (FANCA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 49535-sgFMR1_E3B

    Plasmid

    #157783

    Purpose

    Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPN008

    Plasmid

    #91576

    Purpose

    Express sgRNA targeting human BCL11A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    BCL11A (BCL11A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFD3-ovoD1

    Plasmid

    #111142

    Purpose

    Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

    Insert

    pCFD3-ovoD1 (ovo Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LRG-2.1T-neo-sgROSA

    Plasmid

    #105587

    Purpose

    lentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting rosa26 locus
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pInducer20 CARD8-HA PAM S297A

    Plasmid

    #169983

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, S297A

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN094

    Plasmid

    #91621

    Purpose

    Express sgRNA targeting human GRIN2A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    GRIN2A (GRIN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPN235

    Plasmid

    #91592

    Purpose

    Express sgRNA targeting human CYFIP1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPN234

    Plasmid

    #91591

    Purpose

    Express sgRNA targeting human CYFIP1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPN262

    Plasmid

    #91607

    Purpose

    Express sgRNA targeting human EPHX2

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    EPHX2 (EPHX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 49535-sgFMR1_E17A

    Plasmid

    #157782

    Purpose

    Expresses a sgRNA targeting stop codon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MTK0_001

    Plasmid

    #123924

    Purpose

    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    TU-sgRNA - L1/RE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPN107

    Plasmid

    #91636

    Purpose

    Express sgRNA targeting human KDM4A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    KDM4A (KDM4A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPN106

    Plasmid

    #91635

    Purpose

    Express sgRNA targeting human KDM4A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    KDM4A (KDM4A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LCV2 V-4

    Plasmid

    #84807

    Purpose

    Cas9 with sgRNA targeting Exon 1 of VEGF-A

    Depositor

    Glenn Yiu

    Article

    Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

    Insert

    gRNA targeting VEGF-A (VEGFA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LentiGuide-Puro-CTRLg2 (CG510)

    Plasmid

    #139456

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LCV2 V-5

    Plasmid

    #84808

    Purpose

    Cas9 with sgRNA targeting Exon 1 of VEGF-A

    Depositor

    Glenn Yiu

    Article

    Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

    Insert

    gRNA targeting VEGF-A (VEGFA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLG1-puro-sgATL1-5

    Plasmid

    #109007

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL1-sgRNA #5 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPN060

    Plasmid

    #91593

    Purpose

    Express sgRNA targeting human CYP26B1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CYP26B1 (CYP26B1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPN061

    Plasmid

    #91594

    Purpose

    Express sgRNA targeting human CYP26B1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CYP26B1 (CYP26B1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPN064

    Plasmid

    #91597

    Purpose

    Express sgRNA targeting human DGKI

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DGKI (DGKI Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPN065

    Plasmid

    #91598

    Purpose

    Express sgRNA targeting human DGKI

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DGKI (DGKI Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LentiCRISPRv2-sgSORCS2-G2

    Plasmid

    #118416

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA SORCS2 Human (SORCS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCR1161

    Plasmid

    #111097

    Purpose

    Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCA gRNA (FANCA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCR1110

    Plasmid

    #111096

    Purpose

    Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    BLM gRNA (BLM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPN286

    Plasmid

    #91669

    Purpose

    Express sgRNA targeting human SLC45A1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SLC45A1 (SLC45A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPN287

    Plasmid

    #91670

    Purpose

    Express sgRNA targeting human SLC45A1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SLC45A1 (SLC45A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge