We narrowed to 5,104 results for: Pet2;

1. SARS-CoV-2 Mpro protease (M165I mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204778

   Purpose

   Bacterial expression of Sars Cov 2 MPRO M165I mutant

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   M165I

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. +36GFP-LPETG

   Plasmid

   #71754

   Purpose

   +36GFP-LPETG for expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   (GGS)9_(pos36GFP)_6XHis_LPETG
   

   Use

   Tags

   6XHis and Sortase (LPETG)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET16_Caspase-4_p10

   Plasmid

   #214308

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pC28-LIC-sfGFP-His

   Plasmid

   #165479

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

   Depositor

   Renato Bruni

   Article

   Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

   Type

   Empty backbone

   Use

   Tags

   TEV-GFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Human RNF144A (16-228)

   Plasmid

   #110752

   Purpose

   Bacterial Expression of RNF144A RBR domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   RNF144A (RNF144A Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6BY9 (EHMT1)

   Plasmid

   #119956

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   EHMT1 (EHMT1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBMCS-SortA

   Plasmid

   #21931

   Depositor

   Fuyuhiko Inagaki

   Article

   Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

   Insert

   sortase (srtA Staphylococcus aureus (strain N315))
   

   Use

   Tags

   GB1 and His-tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

8. tUI-HA

   Plasmid

   #122662

   Purpose

   Express tUI-HA protein in E. coli

   Depositor

   Robert Cohen

   Article

   Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   tUI-HA
   

   Use

   Tags

   HA-tag and His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. JMC044-B12

   Plasmid

   #58752

   Purpose

   bacterial expression of PDB 4NW3

   Depositor

   Cheryl Arrowsmith

   Insert

   PDB:4NW3 (KMT2A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available since

   Aug. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. KRASB

    Plasmid

    #25153

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KRASb:M1-K169 (KRAS Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q61H

    Promoter

    Available since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pBEL1994

    Plasmid

    #139898

    Purpose

    Expression plasmid for LetB PLL2 deletion (without TM region)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta229-239, D228G, L240G)-6xHis

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SpyTag003-mClover3

    Plasmid

    #133453

    Purpose

    Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-mClover3
    

    Use

    Tags

    GSGSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBL701

    Plasmid

    #140371

    Purpose

    Expresses TetR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-TetR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETSG

    Plasmid

    #159418

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    polyhistidine (poly-His)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRAF-MutL

    Plasmid

    #86607

    Purpose

    E. coli MutL expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutL
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETNb-nALFA

    Plasmid

    #159988

    Purpose

    For the protein expression with ALFA tag at N-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6BHD (SETDB1)

    Plasmid

    #119955

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    SETDB1 (SETDB1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 190-410

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pRAF-MutH

    Plasmid

    #86609

    Purpose

    E. coli MutH expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutH
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Use

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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