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hsp70I: cPla-mKate2-P2A-EGFP-KDEL

Plasmid

#250915

Purpose

Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in zebrafish live tissue after heat shock

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

cPla2-mKate2-P2A-EGFP-KDEL (pla2g4aa Zebrafish)

Use

D.rerio (zebrafish)

Tags

KDEL on EGFP and mKate2 on cPla2

Promoter

hsp70i

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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UbSYHRE_pDONR201

Plasmid

#249817

Purpose

Encodes UbSYHRE (UBQ-HRE1-GAL4STE12), Gateway compatible as Donor plasmid

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Insert

UBQ-HRE-GAL4STE (ERF71 Budding Yeast, Mustard Weed)

Use

Unspecified

Tags

GAL4-STE12 and UBQ

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hCCDC32-HA

Plasmid

#243756

Purpose

Express human CCDC32 in mammalian cells with HA tag at C terminus.

Depositor

Jingshi Shen

Article

Wan et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Coiled-coil domain-containing protein 32 (CCDC32 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-His-CD86

Plasmid

#232666

Purpose

transient expresses CD86 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD86 (CD86 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-His-ICOSLG

Plasmid

#232661

Purpose

transient expresses ICOSLG in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

ICOSLG (ICOSLG Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-His-CD80

Plasmid

#232665

Purpose

transient expresses CD80 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD80 (CD80 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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px552-sg-gria1-HT

Plasmid

#187652

Purpose

Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Inserts

HaloTag donor sequence
miRFP670

Use

AAV and CRISPR

Tags

N/A

Expression

Mammalian

Promoter

Synapsin

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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UCK2_Deletion_gRNA_1

Plasmid

#204672

Purpose

Dual gRNA plasmid for UCK2 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

uridine-cytidine kinase 2 (UCK2 Human)

Use

Lentiviral

Promoter

U6

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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UCK2_Deletion_gRNA_2

Plasmid

#204673

Purpose

Dual gRNA plasmid for UCK2 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

uridine-cytidine kinase 2 (UCK2 Human)

Use

Lentiviral

Promoter

U6

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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mEos3.2trLATo

Plasmid

#203746

Purpose

Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLATo (Lat Mouse)

Tags

ER translocation signal - mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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