Addgene

We narrowed to 14,644 results for: NOL

Showing: 5461 - 5490 of 14644 results

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pSL2669 (pTnsABf_Vch)

Plasmid

#200897

Purpose

Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsA-TnsB

Use

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

Plasmid

#191145

Purpose

Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3UTR(Bdnf)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB408

Plasmid

#74802

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG

Plasmid

#127300

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19/ER-mChe

Plasmid

#183163

Purpose

Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

ER-mCherry

Use

Tags

25 aa ER signal of Glycine soja kunitz-type tryps…

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pK_P-GAP-ACC1*

Plasmid

#126740

Purpose

yeast plasmid overexpressing ACC1 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acc1*

Use

Tags

Expression

Yeast

Mutation

T3452G

Promoter

GAP

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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eNme2-C TadDE

Plasmid

#193842

Purpose

Express TadDE (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadDE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB543

Plasmid

#74877

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1alpha-rtTA-Antares

Plasmid

#197587

Purpose

Expresses TET-On reverse transactivator labeled with Antares

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

rtTA-P2A-Antares

Use

Tags

Antares

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pShoHELIX-sgRNA_entry (BO3)

Plasmid

#181784

Purpose

Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k

Use

Tags

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG

Plasmid

#127301

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_B358c_attB

Plasmid

#182146

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

ALB_B358c_attB pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_A277c_attB

Plasmid

#182145

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

Alb_A277c_attB pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS2A

Plasmid

#120818

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS2A ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GDI2

Plasmid

#67093

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GDI2 (GDI2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pY027

Plasmid

#84742

Purpose

Expresses huLbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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AA025

Plasmid

#216005

Purpose

Fragmid fragment: (Cas protein) NG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-NG_v1.1 [Sp]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

Plasmid

#191141

Purpose

Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587-W:EGFP

Use

Tags

Expression

Mammalian

Mutation

UAG>UGG (stop>Trp)

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-iRFP-B1-timestamp_A

Plasmid

#159924

Purpose

Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS4A

Plasmid

#120826

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4A ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/UUG-nLuc-3XFLAG

Plasmid

#127302

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCH62 (denAsCas12a-KRAB piggyBac)

Plasmid

#217333

Purpose

piggyBac expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

D908A/E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_TOP-NLuc1.1_L5-L4

Plasmid

#113867

Purpose

Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
NLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

Plasmid

#220224

Purpose

AAV-STARR-seq validation control vector with Venus

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUSE-RapR-Src(KD)-myc

Plasmid

#25934

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-Src (Fkbp1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

This pUSE-RapR-Src(KD)-myc plasmid contains the k…

Promoter

Available since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS4B

Plasmid

#120823

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4B ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_ST18

Plasmid

#64844

Purpose

A lentiviral vector for the expression of mouse ST18 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

ST18 (St18 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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