We narrowed to 15,348 results for: NOL

1. pCAS_gpyrG2

   Plasmid

   #90277

   Purpose

   (also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   gRNA (pyrg2)
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCML 220

   Plasmid

   #98588

   Purpose

   pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEHIShqprtK139A

   Plasmid

   #92403

   Purpose

   hQPRTase harbouring K139A mutation

   Depositor

   James Naismith, Huanting Liu

   Article

   Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

   Insert

   hQPRTase (QPRT Human)
   

   Use

   Tags

   TEV cleacable 6xHis

   Expression

   Bacterial

   Mutation

   K139A

   Promoter

   T7

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8

   Plasmid

   #90285

   Purpose

   BB2 with split pyrG marker for homologous integration with linker AB (negative control)

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   pyrGtrunc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   truncated version of pyrG gene (sequence is from …

   Promoter

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

   Plasmid

   #90286

   Purpose

   BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   pyrGtrunc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   truncated version of pyrG gene (sequence is from …

   Promoter

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

   Plasmid

   #90287

   Purpose

   BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   pyrGtrunc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   truncated version of pyrG gene (sequence is from …

   Promoter

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

   Plasmid

   #90288

   Purpose

   BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   pyrGtrunc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   truncated version of pyrG gene (sequence is from …

   Promoter

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNA

   Plasmid

   #89055

   Purpose

   PV2 gRNA entry vector for CRISPR decoder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNA

   Plasmid

   #89053

   Purpose

   PV1 gRNA entry vector for CRISPR decoder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpA

    Plasmid

    #87576

    Purpose

    PV3 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iRFP720
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpA

    Plasmid

    #87582

    Purpose

    PV3 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBW759_pCAG-PV2-lox2272-GFP-BGHpA

    Plasmid

    #87575

    Purpose

    PV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA

    Plasmid

    #87580

    Purpose

    PV1 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    Neomycin Resistance Gene
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBW807_pCAG-PV4-loxP-mCherry-BGHpA

    Plasmid

    #87583

    Purpose

    PV4 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

    Plasmid

    #87577

    Purpose

    PV4 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mRuby2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBW707_pCAG-PV3-FRT-loxP-mCherry

    Plasmid

    #87571

    Purpose

    PV3 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW705_pCAG-PV1-FRT-lox2272-STOP

    Plasmid

    #87569

    Purpose

    PV1 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    Neomycin Resistance Gene
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW706_pCAG-PV2-lox2272-GFP

    Plasmid

    #87570

    Purpose

    PV2 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ARK2a

    Plasmid

    #81017

    Purpose

    E.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco
    PMD
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    LacUV5 and Trc promoter

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB2336

    Plasmid

    #67541

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAWp7-6

    Plasmid

    #72738

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-[miR-124 + miR-128 + miR-132]

    Depositor

    Timothy Lu

    Article

    Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

    Insert

    UBCp-RFP-CMVp-GFP-[miR-124 + miR-128 + miR-132]
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAWp7-19

    Plasmid

    #72751

    Purpose

    pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]

    Depositor

    Timothy Lu

    Article

    Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

    Insert

    UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAWp7-20

    Plasmid

    #72752

    Purpose

    pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]

    Depositor

    Timothy Lu

    Article

    Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

    Insert

    UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCellFree_G03 RRN3

    Plasmid

    #67112

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RRN3 (RRN3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pCellFree_G03 TFAP4

    Plasmid

    #67073

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAP4 (TFAP4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    P338A

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pET11a+T214V

    Plasmid

    #66134

    Purpose

    Expresses the T214V mutant of CYP119

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pASKt15C+SrtA2woH

    Plasmid

    #65018

    Purpose

    Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108A

    Promoter

    tetracycline promoter/operator

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTH1-SaSrtA-A

    Plasmid

    #64979

    Purpose

    Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108A

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTH1-SaSrtA-K

    Plasmid

    #64978

    Purpose

    Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330A_FokI-1x5

    Plasmid

    #63605

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge