We narrowed to 3,794 results for: Gateway entry

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pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Gateway Cloning

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-SLC7A11 sh926R

Plasmid

#118701

Purpose

entry clone for SLC7A11 insensitive to hairpin TRCN0000288926

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

SLC7A11 (SLC7A11 Human)

Expression

Mammalian

Mutation

wt , SLC7A11 sh926R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pZA8

Plasmid

#158486

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-CBE1

Plasmid

#195367

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

Tags

3xflag

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLEG(R1-R4) #BGV057

Plasmid

#48957

Purpose

Lentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pENTR1A mKate2-hu_ncOGT D554N

Plasmid

#154283

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Tags

2x FLAG and mKate2

Mutation

D554N mutation (Ser/Thr Glycosylation Impaired)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBEG R3-miRNA(ccdB)-L4 #BGV228

Plasmid

#48953

Purpose

To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway Cloning

Promoter

na

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pENTR2b-mVenus

Plasmid

#154898

Purpose

mVenus entry vector

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-mDIXDC1 WT

Plasmid

#61216

Purpose

Entry clone encoding long isoform of mouse DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

DIXDC1 (Dixdc1 Mouse)

Use

Donr clone (gateway)

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221 Cas9-HMGB1

Plasmid

#183198

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-HMGB1

Use

CRISPR and Gateway Cloning

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR and Gateway Cloning

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-RFC5

Plasmid

#183200

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC5

Use

CRISPR and Gateway Cloning

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ221

Plasmid

#132952

Purpose

crRNA entry vector of Prrk1-Not1-HHR with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ675

Plasmid

#132954

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

nucleoplasmin NLS

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ694

Plasmid

#132956

Purpose

crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT 5N5A

Plasmid

#154285

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Tags

2x FLAG and mKate2

Mutation

N322A, N356A, N390A, N424A, N458A mutations (HCF-…

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR4_CELF1

Plasmid

#106098

Purpose

Entry vector for CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Gateway Cloning

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G13-L

Plasmid

#25758

Purpose

Entry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Gateway Cloning

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_TT miRc2 3xFLAG

Plasmid

#83274

Purpose

Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Type

Empty backbone

Use

Gateway Cloning

Tags

3xFLAG

Promoter

TRE tight

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TmiR_G13-L_G12-E

Plasmid

#25763

Purpose

Entry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 and 12 miR-shRNA

Use

Gateway Cloning

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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