We narrowed to 5,135 results for: codon optimized

1. pHS1801

   Plasmid

   #205966

   Purpose

   JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH proteins and CRISPR array
   

   Expression

   Bacterial

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS1232

   Plasmid

   #205969

   Purpose

   MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5-HNH proteins and CRISPR array
   

   Expression

   Bacterial

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

   Plasmid

   #194897

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_WT
   U6-crRNA-scramble
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT-TO-FH-Nsp2

   Plasmid

   #157683

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp2 (ORF1ab SARS-CoV-2)
   

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. NSP14-Flag

   Plasmid

   #210346

   Purpose

   Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-FH-Nsp5

   Plasmid

   #157686

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp5 (ORF1ab SARS-CoV-2)
   

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-32a(+)-TGFB3m

   Plasmid

   #120289

   Purpose

   Produce human recombinant mTGFB3 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β3
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-TO-FH-Orf6

   Plasmid

   #157727

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Orf6 (ORF6 SARS-CoV-2)
   

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pYPQ239

    Plasmid

    #108859

    Purpose

    FnCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    FnCpf1
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Cpf1 is rice codon optimized

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-mCherry-C1-iCoChR++

    Plasmid

    #98173

    Purpose

    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iCoChR++ gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.kc1-HSD17B10

    Plasmid

    #164076

    Purpose

    Human codon optimized HSD17B10. Expression is controlled by doxycycline.

    Depositor

    David Sabatini

    Article

    Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    HSD17B10 (HSD17B10 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-Nsp8-HF

    Plasmid

    #157696

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-HF (ORF1ab SARS-CoV-2)
    

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. p-mCherry-C1-Phobos

    Plasmid

    #98166

    Purpose

    blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-mCherry-C1-Aurora

    Plasmid

    #98167

    Purpose

    red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT3035

    Plasmid

    #122543

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_lmn-1::CemOrange2 (lmn-1 Nematode, Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ165

    Plasmid

    #109327

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    Promoter EC1.2 enhancer fused to EC1.1 promoter

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2-CAG-mScarlet-I-Cdt1

    Plasmid

    #191100

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    mScarlet-I-Cdt1 (30-120)
    

    Use

    AAV and Synthetic Biology

    Tags

    mScarlet-I fused to residues 30-120 of human Cdt1…

    Expression

    Mammalian

    Mutation

    Optimized to human codon usage

    Promoter

    CMV enhancer and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

    Plasmid

    #149507

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence, p.LL256-257AA

    Promoter

    CMV

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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