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pAAV-2Aneo v2

Plasmid

#80033

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Article

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI4

Plasmid

#42528

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

WIPI4 (WDR45 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pTpPuc3

Plasmid

#62864

Purpose

Episome vector for Thalassiosira pseudonana (pUC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for thalassios…

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS15

Plasmid

#99326

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS15 (PIK3R4 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
PARP2-c098

Plasmid

#216873

Purpose

Bacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

Insert

PARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hRIP3 dRHIM

Plasmid

#208788

Purpose

Inducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

RIP3 (RIPK3 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

RHIM domain is removed

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

Plasmid

#174424

Purpose

Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(Thrombin)EGFP(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLC133

Plasmid

#198639

Purpose

Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

dCas9

Tags

3x-FLAG

Expression

Bacterial

Promoter

pTet

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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CRT-GFP pcDNA3.1

Plasmid

#80978

Purpose

mammalian expression of CRT-GFP

Depositor

Peter Steyger

Article

Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

Insert

CRT (Calr Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

no KDEL

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
PB-Antares2

Plasmid

#120868

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-Antares2

Expression

Mammalian

Mutation

Antares2 amplicon

Promoter

CAG Promoter

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-RapR-FAK-YM

Plasmid

#25928

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse, Human)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Myc-eIF3f

Plasmid

#35975

Depositor

Susana Valente

Article

Valente et al Proc Natl Acad Sci U S A. 2009 Mar 17;10

Insert

eukaryotic translation initiation factor 3, subunit F (EIF3F Human)

Tags

Myc

Expression

Mammalian

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pUC118-mCtnnb1-FLIP-Puro

Plasmid

#84540

Purpose

CRISPR - FLIP targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAPcrRNA_Lmo

Plasmid

#126501

Purpose

L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shSMAD4 #2

Plasmid

#83091

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLmoCascade-IL1RNcrRNA

Plasmid

#126487

Purpose

Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo IL1RN cr03

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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All-in-one split-ADAR2

Plasmid

#170136

Purpose

AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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RapR-Shp2-mVenus-flag

Plasmid

#188655

Purpose

Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Shp2

Tags

Flag and mVenus

Expression

Mammalian

Mutation

iFKBP insertion at V406 with deletion of residues…

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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FabF (KASII) pet28 N-terminal His Tag

Plasmid

#75017

Purpose

Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

FabF (fabF E. coli)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only
BoxB-MS2 adRNA (RAB7A-ACG site)

Plasmid

#170155

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(ACG)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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