We narrowed to 14,937 results for: pET

1. pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)

   Plasmid

   #117908

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. HOXA1 (murine) HIS-tag pET

   Plasmid

   #8556

   Depositor

   Corey Largman

   Insert

   HOXA1 (Hoxa1 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   clone contains 130 AA including the homeodomain, …

   Promoter

   Available since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pF711-pET28a-Hs-delta25-MCAD-NHis wt

   Plasmid

   #85112

   Purpose

   expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta25) MCAD (ACADM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pF708-pET28a-Hs-delta38-METTL20-NHis wt

   Plasmid

   #85109

   Purpose

   expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta38) METTL20 (METTL20 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPV00605 pET21GG2-His(6)-BECN(1-265)

   Plasmid

   #136686

   Purpose

   Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 IDR + CC (BECN1 Human)
   

   Use

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207385

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-ybbr-HIS-ddFLN4-Fgß

   Plasmid

   #101719

   Purpose

   wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   ddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b-LOV-ipaA L514K L531E

   Plasmid

   #40253

   Depositor

   Brian Kuhlman

   Article

   Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

   Insert

   LOV-ipaA L514K L531E
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L514K; L531E; Jα helix region hybridized (AsLOV2 …

   Promoter

   T7

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

9. human KANK1 Ankyrin Repeats pET151

   Plasmid

   #164053

   Purpose

   Expresses human KANK1 Ank domain in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HIV-1 integrase F185H/C280S in pET15b

    Plasmid

    #61672

    Purpose

    HIV-1 integrase F185H/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185H/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    T7 promotor

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET-3a-His-MBP-TEV-VC_Cas1_WT

    Plasmid

    #120364

    Purpose

    Expresses wildtype V-C Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VC_Cas1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-3a-His-MBP-TEV-VD_Cas1_WT

    Plasmid

    #120365

    Purpose

    Expresses wildtype V-D Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_Cas1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FL MALT1 Catalytic Mutant in pET29b-Strep

    Plasmid

    #48969

    Purpose

    Expresses Full length catalytic mutant MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Catalytic Mutant C464A

    Promoter

    T7

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

14. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. HOXB7 (human) HIS-tag pET

    Plasmid

    #8538

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pET-45b-NFYC-IDR-mEGFP

    Plasmid

    #185015

    Purpose

    Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    NFYC IDR (Nfyc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

    Plasmid

    #117911

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A & H45Q on eDHFR

    Promoter

    T7

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29-hMIGA2(306-593)-6xhis

    Plasmid

    #192837

    Purpose

    Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HOXB1 (human) HIS-tag pET

    Plasmid

    #8520

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB1 (HOXB1 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    PCR from cDNA clone

    Promoter

    Available since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pET-28a-His6-SUMO-VAMP8

    Plasmid

    #135553

    Purpose

    Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP8
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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