We narrowed to 6,241 results for: nls

1. pMAL716

   Plasmid

   #169150

   Purpose

   Encodes OptoQ-AMP4

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLSLZ.D.R

   Plasmid

   #51510

   Purpose

   Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   NLS

   Promoter

   2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR1055

   Plasmid

   #71823

   Purpose

   Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
   

   Use

   CRISPR

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFTK056

   Plasmid

   #171328

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   SpCas9-NLS
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCNPpo6-H98A

   Plasmid

   #46087

   Depositor

   Raymond Monnat

   Article

   Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

   Insert

   PpoI H98A
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   catalytically inactive H98A variant

   Promoter

   CMV

   Available Since

   June 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK Smad3NL Flag

   Plasmid

   #14828

   Depositor

   Rik Derynck

   Article

   Zhang et al Curr Biol. 1997 Apr 1. 7(4):270-6.

   Insert

   Smad3 NL (SMAD3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   amino acids 1-210

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pZLSLD.R

   Plasmid

   #51511

   Purpose

   Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   NLS

   Promoter

   2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLSLGUS

   Plasmid

   #51517

   Purpose

   pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   GUS
   3kb filler sequence
   

   Use

   Plant t-dna plasmid

   Tags

   NLS and flag

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL1166

   Plasmid

   #129520

   Purpose

   pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   3XFLAG-NLS

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFTK047

    Plasmid

    #171319

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    DsRed-NLS fluorescent reporter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFTK044

    Plasmid

    #171316

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    eGFP-NLS fluorescent reporter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. BII-ChPtinGW

    Plasmid

    #133377

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFTK074

    Plasmid

    #171346

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    VPR -NLS AD (for fusion)
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFTK071

    Plasmid

    #171343

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    eGPF-NLS fluorescent reporter (for fusion)
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFTK070

    Plasmid

    #171342

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    eYFP-NLS fluorescent reporter (for fusion)
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFTK069

    Plasmid

    #171341

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    eGFP-NLS fluorescent reporter (for fusion)
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFTK057

    Plasmid

    #171329

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    dSpCas9(m4)-VPR-NLS
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS42K_PCP-VP64_B-PP7

    Plasmid

    #177157

    Purpose

    Expresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cells

    Depositor

    Gautam Dantas

    Article

    Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

    Inserts

    PCP-VP64
    B-PP7
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    ADH1 promoter and SNR52 promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS2-ecDHFR

    Plasmid

    #65859

    Purpose

    Expresses RNA-binding protein MS2 with eDHFR fusion tag

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

    Inserts

    MS2
    E. coli Dihydrofolate Reductase (folA )
    

    Tags

    HA and NLS

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pSL1103

    Plasmid

    #129526

    Purpose

    pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    dSpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    NLS, 1xHA

    Available Since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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