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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 3721 - 3740 of 18824 results

  7. NLS-tdPCP-CIBN

    Plasmid
    #183937
    Purpose
    PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure
    Depositor
    Karsten Rippe
    Publication
    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdPCP-CIBN (CIB1 Mustard Weed, Synthetic)
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV (TATA box removed)
    Available since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183937-plasmid-map-sequence-id-364100

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  9. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid
    #60492
    Purpose
    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm
    Depositor
    Dorus Gadella
    Publication
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    Tags
    SGFP2 and SGFP2(T65G)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60492-plasmid-map-sequence-id-96974

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  11. XLone-Puro Cas13d P2A eGFP-NLS

    Plasmid
    #155182
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP
    Depositor
    Xiaoping Bao
    Publication
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasRx
    Use
    Unspecified; Piggybac transposon
    Tags
    NLS and P2A eGFP NLS
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155182-plasmid-map-sequence-id-307945

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  12. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

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  13. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid
    #140026
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140026-plasmid-map-sequence-id-275838

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  14. CARMEN

    Plasmid
    #240464
    Purpose
    Compartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER
    Depositor
    Roland Malli
    Publication
    Lischnig et al Br J Pharmacol. 2025 Oct 3. doi: 10.1111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CARMEN
    Expression
    Mammalian
    Available since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    240464-plasmid-map-sequence-id-482332

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  17. 3xnls-cpmTq2-Calcium-lifetime-sensor

    Plasmid
    #129626
    Purpose
    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes
    Depositor
    Dorus Gadella
    Publication
    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xnls-cpmTq2-Calcium-lifetime-sensor
    Tags
    3xNLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    129626-plasmid-map-sequence-id-260197

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  19. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Publication
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    191096-plasmid-map-sequence-id-380123

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Showing: 3721 - 3740 of 18824 results