We narrowed to 13,169 results for: Mpl;

1. pmiRGLO-PTPN18-201

   Plasmid

   #117357

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR PTPN18-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmiRGLO-TSPAN15-201

   Plasmid

   #117375

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR TSPAN15-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmiRGLO-CRTAP-201

   Plasmid

   #117343

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR CRTAP-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmiRGLO-MTMR10-201

   Plasmid

   #117353

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR MTMR10-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmiRGLO-UBE2I-202

   Plasmid

   #117377

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR UBE2I-202
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmiRGLO-PDK2-203

   Plasmid

   #117354

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR PDK2-203
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmiRGLO-SPRED2-201

   Plasmid

   #117366

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR SPRED2-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmiRGLO-TMEM134-201

   Plasmid

   #117371

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR TMEM134-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmiRGLO-UBE2G2-202

   Plasmid

   #117376

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR UBE2G2-202
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmiRGLO-RGS3-201

    Plasmid

    #117359

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR RGS3-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmiRGLO-PLEKHG5-212

    Plasmid

    #117355

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR PLEKHG5-212
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmiRGLO-CAMK2B-209

    Plasmid

    #117342

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR CAMK2B-209
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmiRGLO-ARHGAP4-203

    Plasmid

    #117338

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR ARHGAP4-203
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmiRGLO-SEMA5B-202

    Plasmid

    #117362

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR SEMA5B-202
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmiRGLO-SEMA5B-211

    Plasmid

    #117363

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR SEMA5B-211
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmiRGLO-RPLP1-201

    Plasmid

    #117361

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR RPLP1-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmiRGLO-ASIC1

    Plasmid

    #117339

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR ASIC1
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmiRGLO-ARF1-214

    Plasmid

    #117337

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR ARF1-214
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmiRGLO-LEMD2-201

    Plasmid

    #117349

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR LEMD2-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmiRGLO-PPM1F-201

    Plasmid

    #117334

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR PPM1F-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmiRGLO-GLIS2-201

    Plasmid

    #117331

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR GLIS2-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmiRGLO-CERS2-201

    Plasmid

    #117330

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR CERS2-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC19_T790M_28bp

    Plasmid

    #114721

    Purpose

    28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    c.2369C>T (Substitution, position 2369, C➞T); …

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-6P-1 p37deltaSEP

    Plasmid

    #113501

    Purpose

    Bacterial expression of GST-tagged p37 lacking the SEP domain

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 140-206

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-6P-1 p37delta2-81

    Plasmid

    #113502

    Purpose

    Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 2-81

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFL His-I3

    Plasmid

    #113513

    Purpose

    Expression of His-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19_4xT790M_28bp

    Plasmid

    #114719

    Purpose

    4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    c.2369C>T (Substitution, position 2369, C➞T); …

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a_6HT-TBP6.7(delta C-term)

    Plasmid

    #112731

    Purpose

    This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(delta C-term)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    delta C-term

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. [pSK480] pLJC5 Flag-Depdc5(A)

    Plasmid

    #109338

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant A

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    DIYGD(185-189)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. [pSK481] pLJC5 Flag-Depdc5(B)

    Plasmid

    #109339

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant B

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    EQPLH(371-375)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. [pSK479] pLJC5 Flag-Depdc5(C)

    Plasmid

    #109340

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant C

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    FTPRI(419-423)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCR3-vsv-dCEN-Incenp

    Plasmid

    #108479

    Purpose

    expression of vsv-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

    Insert

    dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    CEN box removed

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSG5-chCAR

    Plasmid

    #87231

    Purpose

    Mammalian expression of chicken CAR

    Depositor

    Fritz Rathjen

    Article

    Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

    Insert

    CXADR (CXADR Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJOG252

    Plasmid

    #80584

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJOG253

    Plasmid

    #80585

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge