We narrowed to 5,092 results for: codon optimized

1. pMXs-3xFlag-SPNS1

   Plasmid

   #198568

   Purpose

   SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1 with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

   Plasmid

   #216378

   Purpose

   Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

   Depositor

   William Weis

   Article

   Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   HA signal sequence-FLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NSP4-EGFP

   Plasmid

   #210378

   Purpose

   Expresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   NSP4 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. NSP2-Flag

   Plasmid

   #210344

   Purpose

   Expresses SARS-CoV-2 NSP2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSP3C-mCherry

   Plasmid

   #210377

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   NSP3C (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NSP4-FR-2Strep

   Plasmid

   #210316

   Purpose

   Expresses SARS-CoV-2 NSP4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. (B) G418 marker (R) (SBE 137)

   Plasmid

   #195011

   Purpose

   geneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   pXYL+G418+tNOS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXYL

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p202-Syt1-C2A-I248V

   Plasmid

   #201554

   Purpose

   Expresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coli

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Synaptotagmin-1 C2A domain (partial)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Isoleucine 248 to Valine (numbering based…

   Promoter

   RBS

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

   Plasmid

   #201552

   Purpose

   Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Kinesin-1
   

   Use

   Tags

   HaloTag

   Expression

   Insect

   Mutation

   changed lysine 282 to arginine (numbering based o…

   Promoter

   Ac5

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. B-HeFSpCas9-A695Q

    Plasmid

    #207368

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A695Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. B-HeFSpCas9-A926Q

    Plasmid

    #207370

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A926Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. B-evoSpCas9-E526K

    Plasmid

    #207365

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-E526K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. B-evoSpCas9-V495M

    Plasmid

    #207363

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-V495M
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y515N, K526E, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. B-HiFi SpCas9

    Plasmid

    #207362

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1BR9-AtWUSSTOP-Δα3

    Plasmid

    #202056

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    third helix of homeodomain deleted (AA82-102)

    Promoter

    T7 Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. B-HeFSpCas9-A1060R

    Plasmid

    #207372

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A1060R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

    Promoter

    CBh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B-HeFSpCas9-A1003K

    Plasmid

    #207371

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A1003K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

    Promoter

    CBh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B-HeFSpCas9-A848K

    Plasmid

    #207369

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A848K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. B-HeFSpCas9-A661R

    Plasmid

    #207367

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A661R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B-evoSpCas9-Q661R

    Plasmid

    #207366

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-Q661R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge