We narrowed to 4,713 results for: crispr c plasmids
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Plasmid#114090PurposeCAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized MbCas12a
TagsNLS(nucleoplasmin) and 3xHAExpressionMammalianAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVi U6_gRNA CMV_SadCas9-KRAB
Plasmid#214609PurposeAll-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassetteDepositorInsertsgRNA
SadCas9
UseAAVTagsNP NLS and SV40 NLSExpressionMammalianMutationD10A, N580APromoterCMV and U6Available SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
plRL58
Plasmid#166886PurposeOptimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
plRL61
Plasmid#163633PurposeOptimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVExpressionMammalianPromoterhPGK1Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJR82
Plasmid#75027PurposeEGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertEGFP^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized EGFPExpressionWormAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJR83
Plasmid#75028PurposemCherry^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmCherry^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized mCherryExpressionWormAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GSX2-tTA-BleoR
Plasmid#161748PurposeTet-ON tTA plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertAdvanced tTA
UseCRISPRExpressionMammalianPromoterendogenous GSX2 promoterAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)
Plasmid#140001PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9-NG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCAGAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)
Plasmid#139998PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCAGAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)
Plasmid#140000PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFPDepositorInserthuman codon optimized CBE4max xCas9(3.7) with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…PromoterCAGAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSynapsin-PE2-Cterminal-lenti
Plasmid#135956PurposeNeuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) C-terminal
UseLentiviralExpressionMammalianMutationMMLV RT(D200N, T306K, W313F, T330P, L603W)PromoterhSynapsinAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast
Plasmid#239972PurposeUpdated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloningDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-3-FT-ENGRAM
Plasmid#179158PurposepiggyBac plasmid for 3'-FT ENGRAM recorderDepositorInsertCsy4
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterminPAvailable SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT_PCaspase_PCi_PC-σ
Plasmid#214040PurposeBacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceumDepositorInsertSAVED-CHAT-Pcaspase-Pci-PC-σ
ExpressionBacterialMutationWTPromoterlacUV5 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
SIN-CMV-Ca9-WPRE
Plasmid#87874PurposeTransfer plasmid for the production of lentiviral vectorsDepositorInsertspCas9
UseLentiviralTagsnlsMutationHuman codon-optimizedPromoterCMVAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX459-TMEM217-gRNA#2
Plasmid#249418PurposepX459 plasmid containing gRNA#2 targeting the end of the coding exon of human TMEM217DepositorInsertTMEM217 (TMEM217 Human)
UseCRISPRAvailable SinceFeb. 25, 2026AvailabilityAcademic Institutions and Nonprofits only -
pML107-KAE1
Plasmid#232891PurposePlasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.DepositorAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-SAM50
Plasmid#232895PurposePlasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.DepositorAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-ARB1
Plasmid#232888PurposePlasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only