Addgene

We narrowed to 7,226 results for: trac

Showing: 3741 - 3760 of 7226 results

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XE129 beta2AR Fz1-pcDNA3.1

Plasmid

#16795

Depositor

Randall Moon

Article

Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

Insert

beta 2 AR Fz1 (Fzd1, Adrb2 Rat)

Use

Xenopus expression

Tags

Expression

Mutation

Chimera of beta 2 adrenergic receptor (extracellu…

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
CLV1 L2_pECIA2

Plasmid

#115100

Purpose

Bait vector CLV1 L2_pECIA2 should be used with prey vector CLV1 L2_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G75820 (CLV1 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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GSO2/EDA23 X079_pECIA2

Plasmid

#115111

Purpose

Bait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G44700 (GSO2 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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BRI1 BRI1_pECIA2

Plasmid

#115086

Purpose

Bait vector BRI1 BRI1_pECIA2 should be used with prey vector BRI1 BRI1_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G39400 (BRI1 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RPK2 X042_pECIA2

Plasmid

#115135

Purpose

Bait vector RPK2 X042_pECIA2 should be used with prey vector RPK2 X042_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G02130 (RPK2 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK1 A6_pECIA2

Plasmid

#114967

Purpose

Bait vector SERK1 A6_pECIA2 should be used with prey vector SERK1 A6_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G71830 (SERK1 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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CLV1 L2_pECIA14

Plasmid

#114900

Purpose

Prey vector CLV1 L2_pECIA14 should be used with bait vector CLV1 L2_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G75820 (CLV1 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK3/BAK1 BAKNcFus_pECIA14

Plasmid

#114775

Purpose

Prey vector SERK3/BAK1 BAKNcFus_pECIA14 should be used with bait vector SERK3/BAK1 BAKNcFus_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G33430 (BAK1 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK1 A6_pECIA14

Plasmid

#114767

Purpose

Prey vector SERK1 A6_pECIA14 should be used with bait vector SERK1 A6_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G71830 (SERK1 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-gamma2S/deltaIL

Plasmid

#119730

Purpose

GABAA receptor expression (chimeric rat gamma2 short subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat delta subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRG2/GABRD (Gabrg2, Gabrd Rat)

Use

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00274

Plasmid

#34402

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1856 (ttk Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR V2 Neo sgITGB1_3

Plasmid

#233250

Purpose

Knock out of murine ITGB1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ITGB1 (Itgb1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 Neo sgITGB1_2

Plasmid

#233249

Purpose

Knock out of murine ITGB1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ITGB1 (Itgb1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-Cherry in pmCherryN1

Plasmid

#187291

Purpose

Express pmCherry-IL2-AH-deltaC2

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2

Promoter

CMV

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbleo-TGFa-EREG-ScNeo

Plasmid

#209915

Purpose

To express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreen

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-EREG-ScNeo (EREG, TGFA Human)

Use

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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CRaf-IRES-pErk reporter

Plasmid

#226170

Purpose

Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)

Use

Tags

3xFlag-tdMCP and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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HEK4-53bp-tag-insertion-pegRNA

Plasmid

#227673

Purpose

PegRNA for 53 bp insertion

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

SpCas9 sgRNA scaffold (EPHA3 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-FMR1-CGG-BsmBI

Plasmid

#222966

Purpose

0CGG with BsmBI site for inserting CGG repeats

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)

Use

CRISPR

Tags

Expression

Mutation

CGG repeats deleted from 5' UTR, BsmBI site …

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD80-ST3-His

Plasmid

#210665

Purpose

Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)

Use

Tags

Histag and Spytag003

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo

Plasmid

#222637

Purpose

Ezrin activation reporter, T567 phosphorylation deficient

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

T567A

Promoter

EF1a

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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