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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,609 results for: trac

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Showing: 3741 - 3760 of 7609 results

  1. Epac-S-H325

    Plasmid
    #228249
    Purpose
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228249-plasmid-map-sequence-id-451794

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  5. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177360
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Promoter
    Synapsine promoter and U6 promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177360-plasmid-map-sequence-id-353669

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  7. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  8. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

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  12. pBGE2.0

    Plasmid
    #165987
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165987-plasmid-map-sequence-id-375875

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  14. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid
    #141100
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    Use
    Donor template
    Available Since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141100-plasmid-map-sequence-id-292288

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  15. pJET-osr1-KI-donor

    Plasmid
    #184875
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Odd-Skipped Related Transcription Factor 1 5' homology arm
    Odd-Skipped Related Transcription Factor 1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184875-plasmid-map-sequence-id-366330

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  16. pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro)

    Plasmid
    #174632
    Purpose
    Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-GCN4-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174632-plasmid-map-sequence-id-346976

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  20. pcDNA3.1 Flag YFP hNKCC1 C820S (NT573)

    Plasmid
    #49075
    Purpose
    Expresses human NKCC1 C820S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C820S in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    49075-plasmid-map-sequence-id-72203

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Showing: 3741 - 3760 of 7609 results