We narrowed to 7,246 results for: CIT

1. pRK5-LYCHOS (GPR155) 4CA-HA

   Plasmid

   #199677

   Purpose

   Transient expression of LYCHOS (GPR155) C595.604.629.638A mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C595.604.629.638 mutated to A

   Promoter

   CMV

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX LYCHOS-FLAG-TiD

   Plasmid

   #199747

   Purpose

   Lentiviral construct to stably express LYCHOS (GPR155) TurboID

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Tags

   Flag and TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV phSyn1(S)-FlpE-bGHpA

   Plasmid

   #50364

   Purpose

   Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   FlpE recombinase gene
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 LYCHOS (GPR155) ∆Permease-HA

   Plasmid

   #199686

   Purpose

   Transient expression of LYCHOS (GPR155) ∆Permease

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion from amino acid 1-369

   Promoter

   CMV

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5 LYCHOS ∆LED-HA

   Plasmid

   #199682

   Purpose

   Transient expression of LYCHOS (GPR155) ∆LED-HA

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion from amino acid 556-651

   Promoter

   CMV

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-LYCHOS (GPR155) FP>IA-HA

   Plasmid

   #199661

   Purpose

   Transient expression of LYCHOS (GPR155) FP>IA mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F43 and P44 mutated to I and A

   Promoter

   CMV

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pαH-S-GSAS-deltaH69/V70

   Plasmid

   #171744

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variant

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1α1.1-Chronos-GFP

   Plasmid

   #122098

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α (1.1 kb short version)

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE3328

   Plasmid

   #107539

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K607R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 SIRT1-deltaExon2

    Plasmid

    #105671

    Purpose

    Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE3329

    Plasmid

    #107538

    Purpose

    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    hLbCpf1(RR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K595R

    Promoter

    CMV and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE3327

    Plasmid

    #107535

    Purpose

    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    hAsCpf1(RVR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    S542R, K548V, N552R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE3334

    Plasmid

    #107537

    Purpose

    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K582V, N586R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pαH-S-GSAS-B.1.351.v5

    Plasmid

    #173790

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 5

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant version 5) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pαH-S-GSAS-D614G-N501Y

    Plasmid

    #171748

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-N501Y variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pαH-S-GSAS-D614G-E484K

    Plasmid

    #171751

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-E484K variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-E484K variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pαH-S-GSAS-D614G-K417N

    Plasmid

    #171752

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-K417N variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pαH-S-GSAS-D614G-I692V

    Plasmid

    #171746

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-I692V variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-I692V variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pαH-S-GSAS-D614G-Y453F

    Plasmid

    #171745

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-Y453F variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-Y453F variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTE3330

    Plasmid

    #107534

    Purpose

    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    hLbCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge