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We narrowed to 568 results for: CIT

Showing: 1 - 20 of 568 results

  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Addgene plasmids get more citations So what do we find? Lots more citations for the papers with plasmids...editing paper on CRISPR/Cas amassed nearly 2,400 citations in its first four years (1). In addition to publishing...associated with Addgene deposits accumulate more citations than those without Addgene deposits? Even more...depositing a plasmid with Addgene causes a paper to get cited more?   Articles with and without plasmids ...associated with Addgene plasmid deposits are more highly cited, we went to one of the largest bibliographic databases...network of articles to track the total number of citations the paper receives. From the database we pulled...papers published in other years also get many more citations This isn’t the whole story, though. It would ...

  2. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... an important part of being a “good scientific citizen” and making sure you do what you can to help your...instill a culture of rewarding good scientific citizenship in all its different forms as part of graduation...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...Developmental Bioelectricity symposium’s “What is Bioelectricity?” Journal of Bioelectricity ...first introduced to bioelectricity when I attended the Developmental Bioelectricity satellite symposium...resources from the bioelectricity field In addition to sharing tools to study bioelectricity, bioelectric researchers... cell networks. “Developmental bioelectricity is an extremely exciting emerging field, with many implications...interdisciplinary field of biology known as bioelectricity is revealing the important role of bioelectrical...., 2010). One of the challenges of studying bioelectricity is that it can be hard to visualize or perturb...to address these hurdles. Tools for studying bioelectricity Dyes and other technologies are now available...

  4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...broader PAM specificity can target more sites. Created with BioRender.com. Specificity Specificity is the...important factors to consider: site accessibility, specificity, and sensitivity. Site accessibility Site accessibility...evolution to develop Cas9s that have a broader PAM specificity, allowing for the targeting of sites that were...Cas9 sensitivity is often closely related to specificity. Sensitivity is a measure of the on-target activity...measuring the indel frequency at a target site. A specificity ratio can be measured by calculating the on-target...by the off-target activity. Figure 2: Specificity is the measure of off-target activity (left) ...Expanding CRISPR Beyond SpCas9 described the PAM specificity of Cas9s isolated from different organisms and...

  5. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    ...explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation that you can...the original depositor. Our citations feature makes it so that, when you cite a plasmid from Addgene using...rely on the citation number when comparing two plasmids, we recommend reviewing the cited papers to determine...your research interests. We do this by promoting citation, supporting sophisticated search, and curating...curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you use a plasmid from the...the repository, we request that you cite it using both its Addgene ID number as well as the reference ... reference section. Why do this? Not only does citing plasmids from Addgene help others find them later...

  6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ... and Doudna labs have improved the on-target specificity of the Cas9 nuclease with engineered variants...cells and that similar mutations can improve the specificity of Cas9 derived from S. aureus. This means you...homolog of choice and get improved on-target specificity. Design and testing of SpCas9-HF1 Kleinstiver...different than those found in eSpCas9, the effect on specificity was similar; SpCas9-HF1 generated fewer off-target...portions of Cas9 might be important for nuclease specificity. They found that eSpCas9(1.1) and SpCas9-HF1 ...propose that this stalling leads to their enhanced specificity. Additional work revealed that mutations in the...possibilities for Cas proteins With their enhanced specificity, eSpCas9, SpCas9-HF1, and HypaCas9 should enable...

  7. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...Plasmids" from these citing articles. What's this? Simply put, if the authors of a "Citing Article" have deposited...Articles Citing This Plasmid" list increasing your work's visibility Help us improve our citations lists...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...publications, you'll find a link to the list of citing articles under the "Resource Information" heading... be directed to a page listing the articles that cite this plasmid. You can use the dropdown to increase...right of the table to search and filter the list of citing articles. From the article list you can click on...deposited plasmids associated with this "Citing Article" - such as cloning their gene of interest into...

  10. Weighing Reagents Protocol

    Type
    Protocol
    ...Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above the...is best. Check that the balance has the correct capacity for the material that you are weighing by looking... you’re weighing out an amount larger than the capacity of your balance or weighing boat, you can weigh...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1 - Mammalian...- Mammalian Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C)... Expression pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus...Mammalian Expression mCitrine-C1 - Mammalian Expression mCitrine-pBAD - Bacterial Expression YPet 517 530 80 ....g. off to on) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission ... Fusions (Organized by Color) For each table: Excitation and Emission maximum are listed in nm. Brightness...Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

  13. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...vector available to the scientific community. Please cite Moffat et al., Cell 2006 Mar; 124(6):1283-98 (PubMed... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting... media 24 hours after infection. TIP: If viral toxicity is observed in your cell line, you may decrease...

  14. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...References for each serotype can be found below under Citations. AAV2(Y444F) The AAV2(Y444F) serotype is demonstrated...Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When using the AAV2(Y444F...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction.... 2009 Mar;17(3):463-71. PMID: 19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant...Ther . Feb;19(2):293-301. PMID: 21045809 Other citations include: Chen, et al. 2014. Reprogramming adipose... Dev . Sep;26(3):165-76. PMID: 26390090 Other citations include: Scalabrino, et al. 2015. Intravitreal...

  15. CRISPR Guide

    Type
    Collection
    ... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most...targeting multiple genomic locations or enhancing specificity, efficiency, or flexibility of the Cas enzyme...Vectors Engineering the Cas9 Endonuclease CRISPR specificity is determined by both the gRNA sequence and the... design, engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the...many modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s...cleavage of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands...fidelity of Cas9 Researchers have also enhanced specificity by engineering Cas9 into high fidelity Cas9s ...

  16. Chemogenetics AAV Preps

    Type
    Collection
    ...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth...-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth ...-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth ...pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Roth 50476 ...pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine none 5 Roth 50478 pAAV-GFAP-hM3D...element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent...

  17. Antibody Guide

    Type
    Collection
    ... of 10 Antigen Specificity Antibodies are available in two types of antigen specificity: monoclonal, meaning...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a specific... Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling Visualization...variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to antibody..., and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...isotype-specific or even isotype subclass-specific. This specificity can be used to ensure secondary antibodies bind...cost-effective. They are useful when epitope specificity is not a concern or in cases where you may want...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Amara , Susan Ingram Citrate Nanosensors for in vivo measurements of citrate (CitA) Engineering genetically...inverse fluorescent biosensor for citrate (Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric...measurements of citrate concentrations. PLoS One. 2011;6(12):e28245. Uwe Sauer Citrate Direct or inverse...Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine MagFRET: The First Genetically Encoded Fluorescent...Inverse-Response Genetically Encoded Biosensors for Citrate. ACS Cent Sci. 2020 Aug 26;6(8):1441-1450. Robert... reveals a role for pH regulation in hair cell toxicity and function. Dis Model Mech. 2014 Jul;7(7):847...Thomas Knopfel Voltage Detection of single action/excitatory potentials in individual neurons and dendrites...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...many of which detail work to improve the tool’s specificity, orthogonality, and multiplexibility in various...endogenous loci with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR...Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . ...Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature . 523(7561):481-5. PMID: 26098369 Komor...Rationally engineered Cas9 nucleases with improved specificity. Science . 351(6268):84-8. PMID: 26628643 Wang...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...generally contained at lower biosafety levels and elicit relatively low immunological effects in vivo . ...non-dividing cells with a low immune response and low toxicity. Although recombinant AAV does not integrate into...AAV is currently limited by its small packaging capacity (∼4.5 kb including ITRs), though there is a great...interest and effort directed toward expanding this capacity. Traditionally, AAV requires the presence of another...
Showing: 1 - 20 of 568 results