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We narrowed to 503 results for: CIT

Showing: 1 - 20 of 503 results

  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Addgene plasmids get more citations So what do we find? Lots more citations for the papers with plasmids...editing paper on CRISPR/Cas amassed nearly 2,400 citations in its first four years (1). In addition to publishing...associated with Addgene deposits accumulate more citations than those without Addgene deposits? Even more...depositing a plasmid with Addgene causes a paper to get cited more?   Articles with and without plasmids ...associated with Addgene plasmid deposits are more highly cited, we went to one of the largest bibliographic databases...network of articles to track the total number of citations the paper receives. From the database we pulled...papers published in other years also get many more citations This isn’t the whole story, though. It would ...

  2. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... an important part of being a “good scientific citizen” and making sure you do what you can to help your...instill a culture of rewarding good scientific citizenship in all its different forms as part of graduation...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...Developmental Bioelectricity symposium’s “What is Bioelectricity?” Journal of Bioelectricity ...first introduced to bioelectricity when I attended the Developmental Bioelectricity satellite symposium...resources from the bioelectricity field In addition to sharing tools to study bioelectricity, bioelectric researchers... cell networks. “Developmental bioelectricity is an extremely exciting emerging field, with many implications...interdisciplinary field of biology known as bioelectricity is revealing the important role of bioelectrical...., 2010). One of the challenges of studying bioelectricity is that it can be hard to visualize or perturb...to address these hurdles. Tools for studying bioelectricity Dyes and other technologies are now available...

  4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...broader PAM specificity can target more sites. Created with BioRender.com. Specificity Specificity is the...important factors to consider: site accessibility, specificity, and sensitivity. Site accessibility Site accessibility...evolution to develop Cas9s that have a broader PAM specificity, allowing for the targeting of sites that were...Cas9 sensitivity is often closely related to specificity. Sensitivity is a measure of the on-target activity...measuring the indel frequency at a target site. A specificity ratio can be measured by calculating the on-target...by the off-target activity. Figure 2: Specificity is the measure of off-target activity (left) ...Expanding CRISPR Beyond SpCas9 described the PAM specificity of Cas9s isolated from different organisms and...

  5. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    Published
    March 10, 2016, 3:30 p.m.
    ...explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation that you can...the original depositor. Our citations feature makes it so that, when you cite a plasmid from Addgene using...rely on the citation number when comparing two plasmids, we recommend reviewing the cited papers to determine...your research interests. We do this by promoting citation, supporting sophisticated search, and curating...curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you use a plasmid from the...the repository, we request that you cite it using both its Addgene ID number as well as the reference ... reference section. Why do this? Not only does citing plasmids from Addgene help others find them later...

  6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ... and Doudna labs have improved the on-target specificity of the Cas9 nuclease with engineered variants...cells and that similar mutations can improve the specificity of Cas9 derived from S. aureus. This means you...homolog of choice and get improved on-target specificity. Design and testing of SpCas9-HF1 Kleinstiver...different than those found in eSpCas9, the effect on specificity was similar; SpCas9-HF1 generated fewer off-target...portions of Cas9 might be important for nuclease specificity. They found that eSpCas9(1.1) and SpCas9-HF1 ...propose that this stalling leads to their enhanced specificity. Additional work revealed that mutations in the...possibilities for Cas proteins With their enhanced specificity, eSpCas9, SpCas9-HF1, and HypaCas9 should enable...

  7. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...Plasmids" from these citing articles. What's this? Simply put, if the authors of a "Citing Article" have deposited...Articles Citing This Plasmid" list increasing your work's visibility Help us improve our citations lists...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...publications, you'll find a link to the list of citing articles under the "Resource Information" heading... be directed to a page listing the articles that cite this plasmid. You can use the dropdown to increase...right of the table to search and filter the list of citing articles. From the article list you can click on...deposited plasmids associated with this "Citing Article" - such as cloning their gene of interest into...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...speak of excitation and emission spectra. Both spectra possess clear maxima where excitation and emission...laser excites at a wavelength of 488 nm (Table 1), which is near a local maximum of PE's excitation spectrum...laser, however, excites at 561 nm, which is close to the global maximum of PE's excitation spectrum where...signal.   Figure 2: Excitation and emission spectra for PE. The excitation spectrum shows two maxima...the red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around...that APC-Cy7 is excited at a lower efficiency than APC. While APC's global excitation maximum lies around...-Cy7's global excitation maximum is about 750 nm. If we were to use a laser that excites at 750 nm, APC-Cy7...

  11. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...fluorophore primarily depends on the excitation power and the excitation wavelength. A fluorophore is less...bleach when it is excited off the peak wavelength, since it will absorb the excitation light less efficiently...decreasing the power of the excitation light reduces the number of excitation/emission cycles per unit of...acquired at low excitation powers. Note, however, that, at a 14-fold higher excitation power, the photostability...lightbulb or exchanging excitation filters). This avoids substantial changes in excitation power and will help...fluorescent proteins for live cell imaging can be excited many times, thereby producing a large number of...story. The photobleaching rate does not depend on excitation power in a linear fashion. This implies that ...

  12. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ... and thereby provide molecular or structural specificity to the viewer. Finally, modern microscope designs...detector or restricting fluorescence to specific excitation volumes to enhance spatial contrast (i.e. optical...microscopy. Collimated (non-converging or diverging) excitation light exits the microscope objective evenly illuminating... CT scanner. This is accomplished by focusing excitation light to a point in the sample and raster scanning...containing a number of holes rotates through the excitation light path. Each hole corresponds to a different...First, widefield and confocal microscopy project excitation light through the entire axial volume of a sample...signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single focal point. This...

  13. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... to an excited state through the absorption of photons. After a brief elevation in this excited state,...objectives. Most systems can only excite and read a set number of excitation and emission wavelengths, as ... or the difference in wavelength between peak excitation spectrum and emission spectrum. Fluorescent dyes...traditional fluorescent protein tags, such as higher specificity, increased versatility in probe attachment, and...chemical structure, leading to differences in their excitation and emission wavelengths, which are pivotal in...dye is fluorescein isothiocyanate (FITC), with excitation/emission peak at 495/517 nm. FITC binds to an...this include coupling to an antibody, whereby excitation of blue light will indicate where FITC is bound...

  14. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ... they elicit an immune response from the body. Several factors contribute to the immunogenicity of a drug...relative immunogenicity score (Mattei et.al., 2022).    Figure 1: Generation of immunogenicity scores...prediction of drug immunogenicity  The goal of in vitro prediction of immunogenicity is to validate the...dose (Lu et.al., 2020).  Evaluating a drug’s immunogenicity in-vitro helps researchers understand a drug...However, in silico and in vitro assessment of immunogenicity can help evaluate risk of an antibody.  In...drug epitopes likely to be presented by T cells to elicit an immune response. T cell epitopes are found in...commercially available algorithm to evaluate the immunogenicity of therapeutics in silico. These algorithms...

  15. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...autofluorescence, and, in the case of exciting cyan-excitable donors, phototoxicity. Another challenge to using ...using the same wavelength for excitation. The result was the cyan-excitable orange fluorescent protein, ...FPs) can be problematic because excitation light can cause phototoxicity, autofluorescence, and perturb...FRET donor excitation wavelengths overlap with optogenetic initiation wavelengths, excitation of the optogenetic...the following characteristics: No photoexcitation High Ion specificity High sensitivity Easy expression...their use is limited in vivo because they must be excited by external light sources. These external sources...with a red-shifted fluor. Here, NLuc internally excites the fluor through a process known as bioluminescent...

  16. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...average number of citations per year and the number of other manuscripts that heavily cite the paper are ...number of citations highly influenced by this paper. Influence is measured by the number of citations and the... the context of these citations. The number of manuscripts that cite the background, methods, or results...author, total number of citations, and the number of publications that heavily cite, or are highly influenced...other authors who they cite the most), and who the author influences (who cites the author the most). ...is essentially a virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are...studying and gives you a sense of what people are excited about. Following scientists in fields outside your...

  17. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...the toxicity of guides that have 1-bp mismatch off-target sites, short guides had reduced toxicity compared...affected by low on-target efficacy, non-specific toxicity, and off-target effects. The flaws of RNAi screens... was validated via a growth screen and a ricin toxicity screen since essential and non-essential genes...identified  60% of the essential genes. The ricin toxicity screen had a 10% false discovery rate and identified...identified 67% of previously identified ricin toxicity genes. The screen also discovered several genes previously...toxic if they were depleted from the screen. This toxicity was used a proxy for detecting Cas9 cutting. Safe...targeting guides better control for non-specific toxicity than non-targeting guides First, Morgens et al...

  18. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...Addgene. How to cite options using the different PID formats The instructions for how to cite any Addgene ...have used the same plasmid through the “Articles Citing this Plasmid” section. Having IDs linked to these...reproducibility of experiments. Additionally, proper citation of these Addgene IDs in published work allows ...section have been updated to include the option of citing with resolvable compact identifier and RRID formats...this plasmid’s page you will find that the “How to cite this plasmid” section now lists the multiple PIDs...n2t.net/addgene:87155 ; RRID:Addgene_87155).” So when citing, you can copy and paste the text attributing the... Or if in doubt, copy and paste the entire line. Cite plasmid using a resolvable compact identifier and...

  19. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...your particular field of research.   Citations tools DOI Citation Formatter Just enter the DOI of a paper...generating citations for a few papers at a time. Mendeley Mendeley works much like other popular citation managers...academic databases.  Downloading Mendeley Cite lets you cite papers from your Mendeley library in Microsoft...Zotero is also another free citation manager that lets you organize and cite papers. With the Zotero Connector...Chrome browsers with just one click. Papers can be cited in Word, LibreOffice, or Google Docs. Files can ...chart highlights the differences between these two citation managers. If you want more tips on finding and...podcast episode 2 "Essential Grad School Resources: Citation Managers, Programming, & Productivity": This podcast...

  20. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...wavelength than the exciting wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs...switch their color when activated by a specific excitation wavelength. This means that the emission wavelength...George G. Stokes) is the shift in wavelength from excitation to emission. For most FPs, Stokes shift is less...shift is ≥ 100nm. Specifically, these proteins are excited by UV light or blue light and their emission is.... Fluorescent Sensors: These FPs change their excitation/emission behavior upon environmental changes ... in mind when choosing a fluorescent protein Excitation & Emission (ex/em): Each FP has its unique ...peak. Therefore, choose FPs that your system can excite, and detect the emission. For example, if your ...
Showing: 1 - 20 of 503 results