We narrowed to 11 results for: CIT

... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most...targeting multiple genomic locations or enhancing specificity, efficiency, or flexibility of the Cas enzyme...Vectors Engineering the Cas9 Endonuclease CRISPR specificity is determined by both the gRNA sequence and the... design, engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the...many modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s...cleavage of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands...fidelity of Cas9 Researchers have also enhanced specificity by engineering Cas9 into high fidelity Cas9s ...

... of 10 Antigen Specificity Antibodies are available in two types of antigen specificity: monoclonal, meaning...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a specific... Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents Handling Visualization...variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to antibody..., and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...isotype-specific or even isotype subclass-specific. This specificity can be used to ensure secondary antibodies bind...cost-effective. They are useful when epitope specificity is not a concern or in cases where you may want...

...due to their well-established methods, relative simplicity of production, and higher titers. However, the... and E4. These modifications aimed to lower immunogenicity issues observed with first-generation rAdV ...vectors and further increase the transgene packaging capacity up to ~10.5 kb. The deleted E2 and E4 regions ...lines. Second-generation rAdV vectors were shown to elicit less immune response when administered in vivo,...vectors are also referred to as "gutless" or high capacity AdV (HCAdV) vectors. Here, all viral sequences...of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector. pAdEasy-2 does not contain...such as transduction efficiency, tropism and immunogenicity. For example, Ad5/35 and Ad5/26 combine the...

...Chlamydomonas reinhardtii . Channelrhodopsin-1 (ChR1) is excited by blue light and permits nonspecific cation influx...physiological pH and traffics well to the membrane. Excitatory (Depolarizing) ChR Variants The optogenetic toolbox... as light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements...system. Here are some key factors to consider: Excitation or inhibition? First things first: do you want...experiment? Depending on your answer, you'd pick an excitatory or inhibitory opsin, respectively. Color of activation..., … Boyden, E. S. (2014). Independent optical excitation of distinct neural populations. Nat Methods, ...Hegemann, P., & Deisseroth, K. (2011). Neocortical excitation/inhibition balance in information processing ...

...DNA synthesis but further limits the packaging capacity of rAAV vectors to ~2.4 kb. Figure 4: Examples...ability to target specific tissues and cells, and capacity for long-term gene expression. These features ...other viral delivery systems due to their non-pathogenicity, ability to efficiently deliver genes to a wide...brain structure due to their small size, low immunogenicity, and limited ability to spread after injection...capsid is challenging due to its limited packaging capacity, especially if any additional regulatory elements... information. Split AAVs to increase packaging capacity Another strategy to deliver cargo too large to...Engelhardt, J. F. (2001). Expanding AAV packaging capacity with trans-splicing or overlapping vectors: a ...

...-term expression in a host, and exhibit low immunogenicity. This guide contains many viral vector-specific...of interest flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid — contains packaging genes gag...popularity to further enhance both infectivity and specificity. Pseudotyped lentiviral vectors are common in...the target cell population and reduce cellular toxicity. Common Uses of Lentiviral Vectors Due to their...lentiviral vectors. Glossary Term Definition Immunogenicity The ability of a molecule or substance to induce...

...ligand-gated ion channels (LGICs) that can either excite or inhibit neuronal signaling. PSAM-based LGICs...generates light that activates the opsin. The opsin may excite or inhibit the neurons expressing the LMO, depending...promoters in chemogenetics plasmids Promoter Cell Specificity hSyn1, CaMKIIa Neurons GFAP Glia CD68 Microglia...tissue, and this must be considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors...

...stable long-term expression in a host and low immunogenicity. This guide contains many viral vector-specific...shRNA of interest flanked by LTRs; ~8 kb packaging capacity Packaging plasmid — contains packaging genes gag...glycoproteins of HIV generates a retroviral vector with specificity of infection for CD4-expressing cells. Proceedings...and the eventual cause of serious diseases. Immunogenicity The ability of a molecule or substance to induce...

...High-fidelity Cas enzymes increase specificity Dual-nickase approach increases specificity but is less efficient Each...be able to completely avoid them. To increase specificity, consider using an engineered Cas enzyme with...use in the lab, but AAVs exhibits the lowest immunogenicity and are one of the least toxic methods for ...

...Nakayama A toolkit with both high cloning capacity and vector simplicity, compatible with other MoClo, GoldenBraid...

...library screens, cells are infected at a very low multiplicity of infection (MOI) to increase the odds that...