We narrowed to 11 results for: CIT

1. Weighing Reagents Protocol

   Type

   Protocol

   ...Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above the...is best. Check that the balance has the correct capacity for the material that you are weighing by looking... you’re weighing out an amount larger than the capacity of your balance or weighing boat, you can weigh...

2. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

   Type

   Protocol

   ...columns have a finite binding capacity. If your sample exceeds the capacity, divide the sample among multiple...columns. For information about the total binding capacity for your columns, refer to the manufacturer’s ...

3. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...vector available to the scientific community. Please cite Moffat et al., Cell 2006 Mar; 124(6):1283-98 (PubMed... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting... media 24 hours after infection. TIP: If viral toxicity is observed in your cell line, you may decrease...

4. Water Bath Protocol

   Type

   Protocol

   ...bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside the water bath...

5. Ligation Independent Cloning

   Type

   Protocol

   ...the first G on 3'-5' strand from cut site). For simplicity, only the 5' primer is shown here. Note: Use ...

6. Fluorescence Titering Assay

   Type

   Protocol

   ... lentiviral vector allows you to control the multiplicity of infection (MOI) in downstream studies. This...

7. Colony Formation Titering Assay

   Type

   Protocol

   ...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...

8. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...roughly 18-21bp is usually sufficient to give specificity and to also be compatible with a standard PCR...

9. Kit Free RNA Extraction

   Type

   Protocol

   ...#1 ): 4 M Guanidinium Thiocyanate 25 mM Sodium Citrate, pH 7.0 0.5% (wt/vol) N-laurosylsarcosine (Sarkosyl...

10. AAV Production in HEK293 Cells

    Type

    Protocol

    ...post transfection. Changing the media can reduce toxicity associated with PEI and allow for the addition...

11. CRISPR Library Amplification

    Type

    Protocol

    ...overloaded. If you find that your pellet outstrips the capacity listed in the Maxiprep protocol, scale the reagent...